Table 1. High- and low-affinity binding... | American Society of Hematology

Table 1.

High- and low-affinity binding constants of TPO:c-MPL in the presence or absence of cytokines

Proteins	High-affinity K_D, nM	Low-affinity K_D, nM
TPO:c-MPL	0.15 ± 0.04	1 066 ± 126
TPO:c-MPL + 1 µM IFN-γ	0.05 ± 0.01	3.72 × 10¹⁸*
TPO:c-MPL + 0.2 µM IFN-γ	n.d.	23 592 ± 19 977
TPO:c-MPL + 0.02 µM IFN-γ	n.d.	2 394 ± 593
TPO:c-MPL + 1 µM SCF	0.08 ± 0.01	1 118 ± 412
TPO:c-MPL + 1 µM Flt3L	0.05 ± 0.01	1 556 ± 582

Proteins	High-affinity K_D, nM	Low-affinity K_D, nM
TPO:c-MPL	0.15 ± 0.04	1 066 ± 126
TPO:c-MPL + 1 µM IFN-γ	0.05 ± 0.01	3.72 × 10¹⁸*
TPO:c-MPL + 0.2 µM IFN-γ	n.d.	23 592 ± 19 977
TPO:c-MPL + 0.02 µM IFN-γ	n.d.	2 394 ± 593
TPO:c-MPL + 1 µM SCF	0.08 ± 0.01	1 118 ± 412
TPO:c-MPL + 1 µM Flt3L	0.05 ± 0.01	1 556 ± 582

Data are mean ± SEM.

n.d., not done.

Error is not reported due to the ambiguity of the nonlinear regression.