Platelet aggregation in whole blood in the presence of scaffold protein and Affimer F5
| . | Aggregation, AU . | Area under the curve, U . | ||||
|---|---|---|---|---|---|---|
| Buffer only . | Scaffold . | F5 . | Buffer only . | Scaffold . | F5 . | |
| ADP | 129 ± 23.4 | 124 ± 22.1 | 113 ± 9.3 | 71 ± 14.1 | 66 ± 13.0 | 53 ± 7.5 |
| Collagen | 137 ± 11.9 | 154 ± 24.2 | 144 ± 22.0 | 66 ± 3.6 | 71 ± 15.7 | 62 ± 14.5 |
| . | Aggregation, AU . | Area under the curve, U . | ||||
|---|---|---|---|---|---|---|
| Buffer only . | Scaffold . | F5 . | Buffer only . | Scaffold . | F5 . | |
| ADP | 129 ± 23.4 | 124 ± 22.1 | 113 ± 9.3 | 71 ± 14.1 | 66 ± 13.0 | 53 ± 7.5 |
| Collagen | 137 ± 11.9 | 154 ± 24.2 | 144 ± 22.0 | 66 ± 3.6 | 71 ± 15.7 | 62 ± 14.5 |
Whole blood aggregation was assessed in the presence of Affimer F5 and scaffold control protein (at 10:1 Affimer:fibrinogen molar ratio), using ADP and collagen as agonists, and was quantified as aggregation (expressed in aggregation units [AU]) and area under the curve (measured in U = AU*min, 1 U = 10 AU*min). Data are presented as mean ± SD of 3 independent experiments. Statistical analysis was performed using 1-way ANOVA comparing with buffer only.