Summary of binding affinities and kinetics of Affimer proteins F5 and G2 studied by SPR
| . | Fibrinogen . | Fibrin . | ||||
|---|---|---|---|---|---|---|
| KD ± SD (nM) . | ka ± SD (M−1⋅s−1) . | kd ± SD (s−1) . | KD ± SD (nM) . | ka ± SD (M−1⋅s−1) . | kd ± SD (s−1) . | |
| F5 | 52 ± 1.3 | 3.6 ± 1.3 ×105 | 0.017 ± 0.002 | 58 ± 9.3 | 4.0 ± 0.6 ×105 | 0.023 ± 0.007 |
| G2 | 38 ± 6.1 | 2.7 ± 0.5 ×105 | 0.010 ± 0.001 | 53 ± 8.2 | 2.6 ± 0.7 ×105 | 0.013 ± 0.003 |
| . | Fibrinogen . | Fibrin . | ||||
|---|---|---|---|---|---|---|
| KD ± SD (nM) . | ka ± SD (M−1⋅s−1) . | kd ± SD (s−1) . | KD ± SD (nM) . | ka ± SD (M−1⋅s−1) . | kd ± SD (s−1) . | |
| F5 | 52 ± 1.3 | 3.6 ± 1.3 ×105 | 0.017 ± 0.002 | 58 ± 9.3 | 4.0 ± 0.6 ×105 | 0.023 ± 0.007 |
| G2 | 38 ± 6.1 | 2.7 ± 0.5 ×105 | 0.010 ± 0.001 | 53 ± 8.2 | 2.6 ± 0.7 ×105 | 0.013 ± 0.003 |
Affinity and rate constants for association (ka) and dissociation (kd) were determined using 1:1 Langmuir binding model. Values are means ± SD of 3 independent experiments.