Frequency and abundance of splenic B-cell subpopulations in TriB mice
| Spleen . | Total spleen cells, × 106 . | Mean ± SE . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T1 . | T2 . | T3 . | Follicular B cells . | MZBs . | MZPs . | ||||||||
| Control | 102 ± 19 | 25.8 ± 12.9 | 1.7 ± 0.9 | 21.9 ± 12.3 | 1.5 ± 0.8 | 3.1 ± 1.7 | 0.7 ± 0.4 | 75.1 ± 8.5 | 9.2 ± 2.6 | 67.6 ± 11.5 | 1.1 ± 0.2 | 29.2 ± 10.2 | 0.6 ± 0.4 |
| TriB | 535 ± 42* | 40.3 ± 20.0 | 1.7 ± 1.2 | 8.0 ± 3.7 | 0.3 ± 0.2 | 2.3 ± 1.1 | 0.9 ± 0.6 | 40.0 ± 11.8 | 6.4 ± 3.1 | 52.6 ± 13.9 | 48.8 ± 18.5* | 42.6 ± 11.7 | 28.6 ± 4.6* |
| TriB (GFP+) | 39.3 ± 21.0 | 1.4 ± 1.0 | 6.5 ± 3.2 | 0.2 ± 0.2 | 2.1 ± 1.1 | 0.8 ± 0.6 | 37.9 ± 9.6† | 5.9 ± 3.0 | 52.8 ± 9.7 | 63.0 ± 12.3‡ | 42.6 ± 11.7 | 28.0 ± 4.7‡ | |
| TriB (GFP−) | 41.4 ± 18.3 | 0.3 ± 0.2 | 13.7 ± 6.6 | 0.1 ± 0.1 | 3.2 ± 1.4 | 0.1 ± 0.1 | 40.1 ± 15.7 | 0.5 ± 0.1 | 54 ± 16.0 | 0.6 ± 0.2 | 38.3 ± 12.3 | 0.6 ± 0.3 | |
| Spleen . | Total spleen cells, × 106 . | Mean ± SE . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T1 . | T2 . | T3 . | Follicular B cells . | MZBs . | MZPs . | ||||||||
| Control | 102 ± 19 | 25.8 ± 12.9 | 1.7 ± 0.9 | 21.9 ± 12.3 | 1.5 ± 0.8 | 3.1 ± 1.7 | 0.7 ± 0.4 | 75.1 ± 8.5 | 9.2 ± 2.6 | 67.6 ± 11.5 | 1.1 ± 0.2 | 29.2 ± 10.2 | 0.6 ± 0.4 |
| TriB | 535 ± 42* | 40.3 ± 20.0 | 1.7 ± 1.2 | 8.0 ± 3.7 | 0.3 ± 0.2 | 2.3 ± 1.1 | 0.9 ± 0.6 | 40.0 ± 11.8 | 6.4 ± 3.1 | 52.6 ± 13.9 | 48.8 ± 18.5* | 42.6 ± 11.7 | 28.6 ± 4.6* |
| TriB (GFP+) | 39.3 ± 21.0 | 1.4 ± 1.0 | 6.5 ± 3.2 | 0.2 ± 0.2 | 2.1 ± 1.1 | 0.8 ± 0.6 | 37.9 ± 9.6† | 5.9 ± 3.0 | 52.8 ± 9.7 | 63.0 ± 12.3‡ | 42.6 ± 11.7 | 28.0 ± 4.7‡ | |
| TriB (GFP−) | 41.4 ± 18.3 | 0.3 ± 0.2 | 13.7 ± 6.6 | 0.1 ± 0.1 | 3.2 ± 1.4 | 0.1 ± 0.1 | 40.1 ± 15.7 | 0.5 ± 0.1 | 54 ± 16.0 | 0.6 ± 0.2 | 38.3 ± 12.3 | 0.6 ± 0.3 | |
Frequency of six distinct splenic B-cell populations was determined using flow cytometry. Absolute numbers were calculated by frequency times total spleen cell number. Five TriB and normal mice each, age 4 to 5 mo in both cases, were used. T1, T2, and T3 refer to transitional type 1, 2, and 3 B cells. Absolute numbers were calculated as follows: T1, spleen cell count × single cell percentage × CD19+B220+ percentage × CD21−IgMhigh percentage × AA4.1+CD23− percentage; T2, spleen cell count × single cell percentage × CD19+B220+ percentage × CD21−IgM+ percentage × AA4.1+CD23+ percentage; T3, spleen cell count × single cell percentage × CD19+B220+ percentage × CD21intIgMint percentage × AA4.1+CD23+ percentage; follicular B cells, spleen cell count × single cell percentage × CD19+B220+ percentage × CD21intIgMhigh percentage × AA4.1−CD23+ percentage; MZPs, spleen cell count × single cell percentage × CD19+B220+ percentage × CD21highIgMhigh percentage × AA4.1−CD23+ percentage; MZBs, spleen cell count × single cell percentage × CD19+B220+ percentage × CD21highIgMint percentage × AA4.1−CD23− percentage.
SE, standard error.
P < .01 (control vs TriB).
P < .05.
P < .01 (control vs TriB [GFP+]).