Proportion of normal donors generating WT-1 peptide–specific T-cell responses after stimulation with AAPCs expressing preselected restricting HLA alleles loaded with specific WT-1–derived immunogenic epitopes identified previously by mapping IFNγ+ T-cell responses to a pool of overlapping pentadecapeptides spanning the whole sequence of the WT-1 protein
| Presenting HLA allele . | Sequence identified . | Identified in no. of donors after total pool stimulation on CAMs . | Proportion of responses in healthy donors to the peptide stimulation (%) . | Predicted . | Predicted sequence . | Predicted . | Proportion of responses in healthy donors to the peptide stimulation . | ||
|---|---|---|---|---|---|---|---|---|---|
| Binding index . | Dissociation time . | Binding index . | Dissociation time . | ||||||
| A0201 | (−99)-(−91)THSPTHPPR | 1 | 6/12 (50%) | 3 | 0 | (−99)-(−91)THSPTHPPR | 3 | 0 | 6/12 (50%) |
| (−75)-(−67)AILDFLLLQ | 1 | 8/12 (67%) | 19 | 0.272 | (−78)-(−70)LLAAILDFL | 28 | 225 | 8/12 (67%) | |
| (−47)-(−39)PGCLQQPEQ | 2 | 2/12 (16%) | 0 | 0 | (−45)-(−36)CLQQPEQQGV | 21 | 70 | 2/12 (16%) | |
| (−27)-(−19)KLGAAEASA | 1 | 8/12 (67%) | 19 | 17 | (−27)-(−19)KLGAAEASA | 19 | 17 | 8/12 (67%) | |
| 6-15RDLNALLPAV | 1 | 3/12 (25%) | 18 | 0.2 | 7-15DLNALLPAV | 27 | 12 | 3/12 (25%) | |
| 22-31GGCALPVSGA | 3 | 9/12 (75%) | 13 | 0.003 | 10-18ALLPAVPSL | 33 | 181 | 3/12 (25%) | |
| 38-46LDFAPPGAS | 2 | 8/12 (67%) | 11 | 0 | 37-45VLDFAPPGA | 16 | 4 | 0/12 (0%) | |
| 126-134RMFPNAPYL | 1 | 5/12 (42%) | 22 | 313 | 126-134RMFPNAPYL | 22 | 313 | 5/12 (42%) | |
| 238-246WNQMNLGAT | 2 | 3/12 (25%) | 19 | 0.3 | 235-243CMTWNQMNL | 17 | 1.5 | 0/8 | |
| Total pool | 13/27 (48%) | 8/12 (67%) | |||||||
| A2402 | 239-248NQMNLGATL | 1 | 4/6 (60%) | 10 | 7.2 | 235-243CMTWNQMNL | 10 | 4 | 1/6 (17%) |
| Total pool | 2/6 (33%) | 6/6 (100%) | |||||||
| B0702 | (−125)-(−117)RQRPHPGAL | 1 | 4/8 (50%) | 15 | 40 | (−125)-(−117)RQRPHPGAL | 15 | 40 | 4/8 (50%) |
| (−119)-(−111)GALRNPTAC | 1 | 5/8 (63%) | 2 | 0.3 | (−118)-(−109)ALRNPTACPL | 15 | 120 | 5/8 (63%) | |
| 323-332FMCAYPGCNK | 1 | 3/8 (38%) | 1 | 0.015 | 327-335YPGCNKRYF | 17 | 0.4 | 4/8 (50%) | |
| Total pool | 2/8 (25%) | 3/8 (38%) | |||||||
| Presenting HLA allele . | Sequence identified . | Identified in no. of donors after total pool stimulation on CAMs . | Proportion of responses in healthy donors to the peptide stimulation (%) . | Predicted . | Predicted sequence . | Predicted . | Proportion of responses in healthy donors to the peptide stimulation . | ||
|---|---|---|---|---|---|---|---|---|---|
| Binding index . | Dissociation time . | Binding index . | Dissociation time . | ||||||
| A0201 | (−99)-(−91)THSPTHPPR | 1 | 6/12 (50%) | 3 | 0 | (−99)-(−91)THSPTHPPR | 3 | 0 | 6/12 (50%) |
| (−75)-(−67)AILDFLLLQ | 1 | 8/12 (67%) | 19 | 0.272 | (−78)-(−70)LLAAILDFL | 28 | 225 | 8/12 (67%) | |
| (−47)-(−39)PGCLQQPEQ | 2 | 2/12 (16%) | 0 | 0 | (−45)-(−36)CLQQPEQQGV | 21 | 70 | 2/12 (16%) | |
| (−27)-(−19)KLGAAEASA | 1 | 8/12 (67%) | 19 | 17 | (−27)-(−19)KLGAAEASA | 19 | 17 | 8/12 (67%) | |
| 6-15RDLNALLPAV | 1 | 3/12 (25%) | 18 | 0.2 | 7-15DLNALLPAV | 27 | 12 | 3/12 (25%) | |
| 22-31GGCALPVSGA | 3 | 9/12 (75%) | 13 | 0.003 | 10-18ALLPAVPSL | 33 | 181 | 3/12 (25%) | |
| 38-46LDFAPPGAS | 2 | 8/12 (67%) | 11 | 0 | 37-45VLDFAPPGA | 16 | 4 | 0/12 (0%) | |
| 126-134RMFPNAPYL | 1 | 5/12 (42%) | 22 | 313 | 126-134RMFPNAPYL | 22 | 313 | 5/12 (42%) | |
| 238-246WNQMNLGAT | 2 | 3/12 (25%) | 19 | 0.3 | 235-243CMTWNQMNL | 17 | 1.5 | 0/8 | |
| Total pool | 13/27 (48%) | 8/12 (67%) | |||||||
| A2402 | 239-248NQMNLGATL | 1 | 4/6 (60%) | 10 | 7.2 | 235-243CMTWNQMNL | 10 | 4 | 1/6 (17%) |
| Total pool | 2/6 (33%) | 6/6 (100%) | |||||||
| B0702 | (−125)-(−117)RQRPHPGAL | 1 | 4/8 (50%) | 15 | 40 | (−125)-(−117)RQRPHPGAL | 15 | 40 | 4/8 (50%) |
| (−119)-(−111)GALRNPTAC | 1 | 5/8 (63%) | 2 | 0.3 | (−118)-(−109)ALRNPTACPL | 15 | 120 | 5/8 (63%) | |
| 323-332FMCAYPGCNK | 1 | 3/8 (38%) | 1 | 0.015 | 327-335YPGCNKRYF | 17 | 0.4 | 4/8 (50%) | |
| Total pool | 2/8 (25%) | 3/8 (38%) | |||||||