Leukemocidal activity of defined epitope-specific and HLA-restricted T cells from normal donors sensitized with either autologous EBV-BLCLs or EBV-BLCLs loaded with pooled WT-1 peptides against primary WT-1+ leukemia cells sharing the T cells' restricting HLA allele (P < .001)
| 15-mer no. Containing the dominant epitope . | Sequence identified . | Presenting HLA allele . | Cytotoxic CTL response, % (at a 50:1 effector: stimulator ratio) vs WT-1+ leukemia cells expressing restricting HLA allele . | |
|---|---|---|---|---|
| WT-1 CTLs . | EBV CTLs . | |||
| 1 | (−125)-(−117)RQRPHPGAL | B0702 | 67 | 1 |
| 2 | (−119)-(−111)GALRNPTAC | B0702 | 60 | 2 |
| 4 | (−110)-(−102)PLPHFPPSL | A0201 | 22 | 3 |
| 7 | (−99)-(−91)THSPTHPPR | B4001 | 65 | 0 |
| A0201 | 38 | 3 | ||
| 13 | (−75)-(67)AILDFLLLQ | A0201 | 19 | 5 |
| 20 | (−47)-(39)PGCLQQPEQ | A0201 | 19 | 10 |
| 24-25 | (−27)-(−19)KLGAAEASA | A0201 | 37 | 5 |
| 29-30 | (−8)-(1)ASGSEPQQM | B3501 | 39 | 0 |
| 33 | 6-15RDLNALLPAV | A0201 | 9 | 0 |
| 37 | 22-31GGCALPVSGA | A0201 | 47 | 3 |
| 41 | 38-46LDFAPPGAS | A0201 | 40 | 0 |
| 38-48LDFAPPGASAY | DRB10402 | 40 | 0 | |
| 43 | 46-54SAYGSLGGP | A0201 | 0 | 0 |
| B4001 | 68 | 3 | ||
| 46 | 58-66PAPPPPPPP | A0201 | 0 | 0 |
| 62-63 | 126-134RMFPNAPYL* | A0201 | 25 | 2 |
| 74-75 | 174-182HSFKHEDPM | B3501 | 45 | 5 |
| 83-84 | 209-217CTGSQALLL | A0101 | 33 | 3 |
| 83 | 206-214TDSCTGSQA | B4402 | 56 | 1 |
| 86 | 218-226RTPYSSDNL | B3503 | 48 | 4 |
| C0401 | 48 | 4 | ||
| 87 | 225-233NLYQMTSQLE | A0201 | 0 | 0 |
| 91 | 238-246WNQMNLGAT | A0201 | 19 | 1 |
| C1701 | 16 | 1 | ||
| B3508 | 19 | 4 | ||
| 91-92 | 239-248NQMNLGATL | A2402 | 17 | 1 |
| 91 | 238-248WNQMNLGATLK | DRB11104 | 0 | 0 |
| 235-249CMTWNQMNLGATLKG | DRB10402 | 17 | 1 | |
| 92 | 242-250NLGATLKGV | A0201 | 19 | 2 |
| 99-100 | 269-278GYESDNHTT | A0101 | 33 | 0 |
| 112-113 | 323-332FMCAYPGCNK | B3501 | 45 | 5 |
| 320-334KRPFMCAYPGC | DRB10401 | 5 | 5 | |
| 141 | 436-445NMHQRNHTKL* | A0201 | 0 | 0 |
| B4001 | 72 | 3 | ||
| A2402 | 0 | 0 | ||
| 15-mer no. Containing the dominant epitope . | Sequence identified . | Presenting HLA allele . | Cytotoxic CTL response, % (at a 50:1 effector: stimulator ratio) vs WT-1+ leukemia cells expressing restricting HLA allele . | |
|---|---|---|---|---|
| WT-1 CTLs . | EBV CTLs . | |||
| 1 | (−125)-(−117)RQRPHPGAL | B0702 | 67 | 1 |
| 2 | (−119)-(−111)GALRNPTAC | B0702 | 60 | 2 |
| 4 | (−110)-(−102)PLPHFPPSL | A0201 | 22 | 3 |
| 7 | (−99)-(−91)THSPTHPPR | B4001 | 65 | 0 |
| A0201 | 38 | 3 | ||
| 13 | (−75)-(67)AILDFLLLQ | A0201 | 19 | 5 |
| 20 | (−47)-(39)PGCLQQPEQ | A0201 | 19 | 10 |
| 24-25 | (−27)-(−19)KLGAAEASA | A0201 | 37 | 5 |
| 29-30 | (−8)-(1)ASGSEPQQM | B3501 | 39 | 0 |
| 33 | 6-15RDLNALLPAV | A0201 | 9 | 0 |
| 37 | 22-31GGCALPVSGA | A0201 | 47 | 3 |
| 41 | 38-46LDFAPPGAS | A0201 | 40 | 0 |
| 38-48LDFAPPGASAY | DRB10402 | 40 | 0 | |
| 43 | 46-54SAYGSLGGP | A0201 | 0 | 0 |
| B4001 | 68 | 3 | ||
| 46 | 58-66PAPPPPPPP | A0201 | 0 | 0 |
| 62-63 | 126-134RMFPNAPYL* | A0201 | 25 | 2 |
| 74-75 | 174-182HSFKHEDPM | B3501 | 45 | 5 |
| 83-84 | 209-217CTGSQALLL | A0101 | 33 | 3 |
| 83 | 206-214TDSCTGSQA | B4402 | 56 | 1 |
| 86 | 218-226RTPYSSDNL | B3503 | 48 | 4 |
| C0401 | 48 | 4 | ||
| 87 | 225-233NLYQMTSQLE | A0201 | 0 | 0 |
| 91 | 238-246WNQMNLGAT | A0201 | 19 | 1 |
| C1701 | 16 | 1 | ||
| B3508 | 19 | 4 | ||
| 91-92 | 239-248NQMNLGATL | A2402 | 17 | 1 |
| 91 | 238-248WNQMNLGATLK | DRB11104 | 0 | 0 |
| 235-249CMTWNQMNLGATLKG | DRB10402 | 17 | 1 | |
| 92 | 242-250NLGATLKGV | A0201 | 19 | 2 |
| 99-100 | 269-278GYESDNHTT | A0101 | 33 | 0 |
| 112-113 | 323-332FMCAYPGCNK | B3501 | 45 | 5 |
| 320-334KRPFMCAYPGC | DRB10401 | 5 | 5 | |
| 141 | 436-445NMHQRNHTKL* | A0201 | 0 | 0 |
| B4001 | 72 | 3 | ||
| A2402 | 0 | 0 | ||
Differences between responses of T cells sensitized with WT-1 peptide pool loaded EBVBLCL and those sensitized with EBVBLCL alone, analyzed by paired t test, are significant (P < .001).
Epitopes previously predicted by the computer algorithm or described in the literature.