WT-1–derived immunogenic epitopes identified by mapping the IFNγ responses of T cells after sensitization with the pool of overlapping pentadecapeptides spanning the whole sequence of the WT-1 protein
| Presenting HLA allele . | Sequence identified . | Prediction algorithm . | Cytotoxic CTL response, % (at a 50:1 effector: stimulator ratio) vs: . | ||||
|---|---|---|---|---|---|---|---|
| Binding index . | Disassociation time . | WT-1− allo-APCs with restricting HLA allele . | WT-1+ allo-APCs with restricting HLA allele loaded with WT-1 peptide . | WT-1− leukemia cells . | WT-1+ leukemia cells . | ||
| A0101 | 146-154NQGYSTVTF | 3 | 0.001 | 4 | 15 | ND | ND |
| 209-217CTGSQALLL | 12 | 0.125 | 0 | 26 | 3 | 33 | |
| 238-246WNQMNLGAT | 2 | 0 | 3 | 19 | ND | ND | |
| 242-250NLGATLKGV | 3 | 0.01 | 1 | 17 | ND | ND | |
| 269-278GYESDNHTT | 15 | 1.5 | 0 | 26 | 0 | 33 | |
| 323-332FMCAYPGCNK* | 0 | 0.1 | 2 | 0 | 5 | 0 | |
| A0201 | (−110)-(−102)PLPHFPPSL | 21 | 2 | 1 | 24 | 2 | 22 |
| (−99)-(−91)THSPTHPPR | 3 | 0 | 1 | 21 | 0 | 38 | |
| (−75)-(−67)AILDFLLLQ | 19 | 0.272 | 3 | 17 | 3 | 19 | |
| (−47)-(−39)PGCLQQPEQ | 0 | 0 | 7 | 27 | 5 | 19 | |
| (−27)-(−19)KLGAAEASA | 19 | 17 | 2 | 22 | 10 | 37 | |
| 6-15RDLNALLPAV | 18 | 0.2 | 4 | 31 | 0 | 9 | |
| 22-31GGCALPVSGA | 13 | 0.003 | 3 | 25 | 3 | 47 | |
| 38-46LDFAPPGAS | 11 | 0 | 1 | 62 | 0 | 40 | |
| 46-54SAYGSLGGP* | 14 | 0 | 5 | 31 | 0 | 0 | |
| 58-66PAPPPPPPP* | 5 | 0 | 1 | 18 | 0 | 0 | |
| 126-134RMFPNAPYL† | 22 | 313 | 1 | 52 | 2 | 25 | |
| 225-233NLYQMTSQLE* | 23 | 68 | 3 | 28 | 0 | 0 | |
| 238-246WNQMNLGAT | 19 | 0.3 | 0 | 21 | 1 | 19 | |
| 242-250NLGATLKGV | 24 | 160 | 1 | 14 | 2 | 19 | |
| 390-398RKFSRSDHL | 11 | 0.054 | 1 | 27 | ND | ND | |
| 398-406LKTHTTRTHT | 5 | 0.18 | 1 | 22 | ND | ND | |
| 436-445NMHQRNHTKL* | 20 | 15 | 4 | 32 | 0 | 0 | |
| A0203 | 243-252LGATLKGVAA | 19 | NA | 0 | 21 | ND | ND |
| A2402 | 239-248NQMNLGATL | 10 | 7.2 | 0 | 2 | 1 | 17 |
| 436-445NMHQRNHTKL* | 13 | 0.6 | 13 | 27 | 0 | 0 | |
| A6901 | 246-253TLKGVAAGS | NA | NA | 0 | 57 | ND | ND |
| B0702 | (−125)-(−117)RQRPHPGAL | 15 | 40 | 1 | 53 | 1 | 67 |
| (−119)-(−111)GALRNPTAC | 2 | 0.3 | 5 | 22 | 1 | 60 | |
| A3101 | (−107)-(−99)HFPPSLPPT | NA | 0.01 | 0 | 27 | ND | ND |
| B3501 | (−8)-(−1)ASGSEPQQM | NA | 15 | 3 | 51 | 5 | 39 |
| 135-143PSCLESQPA | NA | 0.075 | 0 | 21 | ND | ND | |
| 174-182HSFKHEDPM | NA | 10 | 3 | 63 | 5 | 45 | |
| 269-278GYESDNHTT | NA | 0.004 | 0 | 23 | ND | ND | |
| 323-332FMCAYPGCNK | NA | 0.01 | 0 | 61 | 5 | 45 | |
| B3503 | 122-130SGQARMFPN | NA | NA | 3 | 41 | ND | ND |
| 218-226RTPYSSDNL | NA | NA | 3 | 31 | 4 | 48 | |
| B3508 | 238-246WNQMNLGAT | NA | NA | 2 | 21 | 4 | 19 |
| B3802 | 206-214TDSCTGSQA | NA | NA | 1 | 53 | ND | ND |
| B3801 | 166-174HHAAQFPNH | 11 | 0.3 | 1 | 17 | ND | ND |
| B3901 | 30-38GAAQWAPVL | 12 | 3 | 0 | 19 | ND | ND |
| B4001 | (−99)-(−91)THSPTHPPR | 3 | 0.02 | 0 | 31 | 3 | 65 |
| 46-54SAYGSLGGP | 1 | 0.002 | 8 | 24 | 3 | 68 | |
| 436-445NMHQRNHTKL | 1 | 0.002 | 1 | 26 | 3 | 72 | |
| B4402 | 202-210CHTPTDSCT | 3 | NA | 7 | 19 | ND | ND |
| 206-214TDSCTGSQA | 2 | NA | 0 | 88 | 1 | 56 | |
| 106-114ACRYGPFGP | 4 | NA | 7 | 23 | ND | ND | |
| B4701 | (−47)-(−37)PGCLQQPEQ | 1 | NA | 1 | 25 | ND | ND |
| B5701 | 6-15RDLNALLPAV | NA | NA | 1 | 22 | ND | ND |
| C0401 | 122-130SGQARMFPN | NA | NA | 3 | 41 | ND | ND |
| C1701 | 238-246WNQMNLGAT | NA | NA | 0 | 7 | 1 | 16 |
| DRB10101 | (−47)-(−37)PGCLQQPEQQG | 8 | NA | 1 | 25 | ND | ND |
| DRB10402 | 38-48LDFAPPGASAY | NA | NA | 1 | 71 | 0 | 40 |
| DRB10402 | 235-249CMTWNQMNLGATLKG | NA | NA | 2 | 15 | 1 | 17 |
| DRB10401 | 320-334KRPFMCAYPGC | 22 | NA | 3 | 0 | 5 | 5 |
| DRB11104 | 238-248WNQMNLGATLK | NA | NA | 2 | 1 | 0 | 0 |
| Presenting HLA allele . | Sequence identified . | Prediction algorithm . | Cytotoxic CTL response, % (at a 50:1 effector: stimulator ratio) vs: . | ||||
|---|---|---|---|---|---|---|---|
| Binding index . | Disassociation time . | WT-1− allo-APCs with restricting HLA allele . | WT-1+ allo-APCs with restricting HLA allele loaded with WT-1 peptide . | WT-1− leukemia cells . | WT-1+ leukemia cells . | ||
| A0101 | 146-154NQGYSTVTF | 3 | 0.001 | 4 | 15 | ND | ND |
| 209-217CTGSQALLL | 12 | 0.125 | 0 | 26 | 3 | 33 | |
| 238-246WNQMNLGAT | 2 | 0 | 3 | 19 | ND | ND | |
| 242-250NLGATLKGV | 3 | 0.01 | 1 | 17 | ND | ND | |
| 269-278GYESDNHTT | 15 | 1.5 | 0 | 26 | 0 | 33 | |
| 323-332FMCAYPGCNK* | 0 | 0.1 | 2 | 0 | 5 | 0 | |
| A0201 | (−110)-(−102)PLPHFPPSL | 21 | 2 | 1 | 24 | 2 | 22 |
| (−99)-(−91)THSPTHPPR | 3 | 0 | 1 | 21 | 0 | 38 | |
| (−75)-(−67)AILDFLLLQ | 19 | 0.272 | 3 | 17 | 3 | 19 | |
| (−47)-(−39)PGCLQQPEQ | 0 | 0 | 7 | 27 | 5 | 19 | |
| (−27)-(−19)KLGAAEASA | 19 | 17 | 2 | 22 | 10 | 37 | |
| 6-15RDLNALLPAV | 18 | 0.2 | 4 | 31 | 0 | 9 | |
| 22-31GGCALPVSGA | 13 | 0.003 | 3 | 25 | 3 | 47 | |
| 38-46LDFAPPGAS | 11 | 0 | 1 | 62 | 0 | 40 | |
| 46-54SAYGSLGGP* | 14 | 0 | 5 | 31 | 0 | 0 | |
| 58-66PAPPPPPPP* | 5 | 0 | 1 | 18 | 0 | 0 | |
| 126-134RMFPNAPYL† | 22 | 313 | 1 | 52 | 2 | 25 | |
| 225-233NLYQMTSQLE* | 23 | 68 | 3 | 28 | 0 | 0 | |
| 238-246WNQMNLGAT | 19 | 0.3 | 0 | 21 | 1 | 19 | |
| 242-250NLGATLKGV | 24 | 160 | 1 | 14 | 2 | 19 | |
| 390-398RKFSRSDHL | 11 | 0.054 | 1 | 27 | ND | ND | |
| 398-406LKTHTTRTHT | 5 | 0.18 | 1 | 22 | ND | ND | |
| 436-445NMHQRNHTKL* | 20 | 15 | 4 | 32 | 0 | 0 | |
| A0203 | 243-252LGATLKGVAA | 19 | NA | 0 | 21 | ND | ND |
| A2402 | 239-248NQMNLGATL | 10 | 7.2 | 0 | 2 | 1 | 17 |
| 436-445NMHQRNHTKL* | 13 | 0.6 | 13 | 27 | 0 | 0 | |
| A6901 | 246-253TLKGVAAGS | NA | NA | 0 | 57 | ND | ND |
| B0702 | (−125)-(−117)RQRPHPGAL | 15 | 40 | 1 | 53 | 1 | 67 |
| (−119)-(−111)GALRNPTAC | 2 | 0.3 | 5 | 22 | 1 | 60 | |
| A3101 | (−107)-(−99)HFPPSLPPT | NA | 0.01 | 0 | 27 | ND | ND |
| B3501 | (−8)-(−1)ASGSEPQQM | NA | 15 | 3 | 51 | 5 | 39 |
| 135-143PSCLESQPA | NA | 0.075 | 0 | 21 | ND | ND | |
| 174-182HSFKHEDPM | NA | 10 | 3 | 63 | 5 | 45 | |
| 269-278GYESDNHTT | NA | 0.004 | 0 | 23 | ND | ND | |
| 323-332FMCAYPGCNK | NA | 0.01 | 0 | 61 | 5 | 45 | |
| B3503 | 122-130SGQARMFPN | NA | NA | 3 | 41 | ND | ND |
| 218-226RTPYSSDNL | NA | NA | 3 | 31 | 4 | 48 | |
| B3508 | 238-246WNQMNLGAT | NA | NA | 2 | 21 | 4 | 19 |
| B3802 | 206-214TDSCTGSQA | NA | NA | 1 | 53 | ND | ND |
| B3801 | 166-174HHAAQFPNH | 11 | 0.3 | 1 | 17 | ND | ND |
| B3901 | 30-38GAAQWAPVL | 12 | 3 | 0 | 19 | ND | ND |
| B4001 | (−99)-(−91)THSPTHPPR | 3 | 0.02 | 0 | 31 | 3 | 65 |
| 46-54SAYGSLGGP | 1 | 0.002 | 8 | 24 | 3 | 68 | |
| 436-445NMHQRNHTKL | 1 | 0.002 | 1 | 26 | 3 | 72 | |
| B4402 | 202-210CHTPTDSCT | 3 | NA | 7 | 19 | ND | ND |
| 206-214TDSCTGSQA | 2 | NA | 0 | 88 | 1 | 56 | |
| 106-114ACRYGPFGP | 4 | NA | 7 | 23 | ND | ND | |
| B4701 | (−47)-(−37)PGCLQQPEQ | 1 | NA | 1 | 25 | ND | ND |
| B5701 | 6-15RDLNALLPAV | NA | NA | 1 | 22 | ND | ND |
| C0401 | 122-130SGQARMFPN | NA | NA | 3 | 41 | ND | ND |
| C1701 | 238-246WNQMNLGAT | NA | NA | 0 | 7 | 1 | 16 |
| DRB10101 | (−47)-(−37)PGCLQQPEQQG | 8 | NA | 1 | 25 | ND | ND |
| DRB10402 | 38-48LDFAPPGASAY | NA | NA | 1 | 71 | 0 | 40 |
| DRB10402 | 235-249CMTWNQMNLGATLKG | NA | NA | 2 | 15 | 1 | 17 |
| DRB10401 | 320-334KRPFMCAYPGC | 22 | NA | 3 | 0 | 5 | 5 |
| DRB11104 | 238-248WNQMNLGATLK | NA | NA | 2 | 1 | 0 | 0 |