Table 1 WT-1 − derived immunogenic... | American Society of Hematology

Table 1

WT-1⁻derived immunogenic epitopes identified by mapping the IFNγ responses of T cells after sensitization with the pool of overlapping pentadecapeptides spanning the whole sequence of the WT-1 protein

15-mer no. containing the dominant epitope	Sequence identified	Presenting HLA allele	IFNγ response of T cells, % IFNγ⁺ cells		Cytotoxic CTL response, % (at a 50:1 effector: stimulator ratio) vs:
15-mer no. containing the dominant epitope	Sequence identified	Presenting HLA allele	No WT-1 peptide	WT-1 peptide loaded	WT-1⁻ autologous APCs	WT-1 peptide–loaded autologous APCs	WT-1⁻ leukemia cells	WT-1⁺ leukemia cells*
1	_{(−125)-(−117)}RQRPHPGAL	B0702	0.9	11.3	0	27	1	67
2	_{(−119)-(−111)}GALRNPTAC	B0702	0.5	14.0	0	30	1	60
4	_{(−110)-(−102)}PLPHFPPSL	A0201	0.98	5.75	0	30	2	22
5	_{(−107)-(−99)}HFPPSLPPT	A3101	0.73	4.82	0	42	ND	ND
7	_{(−99)-(−91)}THSPTHPPR	B4001	1.5	12.8	0	45	3	65
		A0201	0.4	5	2	50	0	38
13	_(−75)-(67)AILDFLLLQ	A0201	0.61	5.07	0	18	3	19
20	_(−47)-(39)PGCLQQPEQ	A0201	0.2	3.67	6	54	5	19
		B4701	0.5	4.6	6	54	ND	ND
	_{(−47)-(−37)}PGCLQQPEQQG	DRB₁0101	0.33	3.1	6	54	ND	ND
24-25	_{(−27)-(−19)}KLGAAEASA	A0201	1.05	4.48	3	41	10	37
29-30	_(−8)-(1)ASGSEPQQM	B3501	0.07	1.0	5	73	5	39
33	_6-15RDLNALLPAV	A0201	1.1	11.0	2	51	0	9
		B5701	0.19	1.24	3	44	ND	ND
37	_22-31GGCALPVSGA	A0201	0.07	0.9	8	32	3	47
39	_30-38GAAQWAPVL	B3901	0.1	1.3	2	31	ND	ND
41	_38-46LDFAPPGAS	A0201	0.2	4.18	0	73	0	40
	_38-48LDFAPPGASAY	DRB₁0402	0.2	1.41	0	73	0	40
43	_46-54SAYGSLGGP	A0201	1.2	6.46	2	51	0	0
		B4001	1.09	6.84	2	41	3	68
46	_58-66PAPPPPPPP	A0201	1.15	6.69	2	40	0	0
58	_106-114ACRYGPFGP	B4402	0.92	5.65	8	46	ND	ND
62	_122-130SGQARMFPN	B3503	0.78	2.0	0	84	ND	ND
		C0401	0.78	2.0	0	84	ND	ND
62-63	_126-134RMFPNAPYL†	A0201	0.52	2.17	3	41	2	25
65-66	_135-143PSCLESQPA	B3501	0.07	0.61	0	35	ND	ND
68	_146-154NQGYSTVTF	A0101	0.92	4.0	2	19	ND	ND
73	_166-174HHAAQFPNH	B3801	0.81	3.14	0	26	ND	ND
74-75	_174-182HSFKHEDPM	B3501	1.3	18.0	0	50	5	45
82	_202-210CHTPTDSCT	B4402	1.02	3.77	8	37	ND	ND
83-84	_209-217CTGSQALLL	A0101	0.03	0.29	0	21	3	33
83	_206-214TDSCTGSQA	B3802	0.71	4.02	0	88	ND	ND
		B4402	1.01	4.2	1	36	1	56
86	_218-226RTPYSSDNL	B3503	0.84	3.0	0	84	4	48
		C0401	0.84	3.0	0	84	4	48
87	_225-233NLYQMTSQLE	A0201	0.13	0.9	3	87	0	0
91	_238-246WNQMNLGAT	A0201	1.34	8.0	0	18	1	19
		C1701	2.1	12.0	0	10	1	16
		A0101	2.1	7.31	0	26	ND	ND
		B3508	1.23	5.0	0	18	4	19
91-92	_239-248NQMNLGATL	A2402	0.02	0.14	4	9	1	17
91	_238-248WNQMNLGATLK	DRB₁1104	0.59	6.0	0	8	0	0
	_235-249CMTWNQMNLGATLKG	DRB₁0402	0.07	0.53	4	16	1	17
92	_242-250NLGATLKGV	A0101	0.32	1.83	2	19	ND	ND
		A0201	0.06	0.75	1	18	2	19
92-93	_243-252LGATLKGVAA	A0203	0.54	2.1	0	35	ND	ND
93	_246-253TLKGVAAGS	A6901	0.09	1.85	4	80	ND	ND
99-100	_269-278GYESDNHTT	A0101	0.12	2.43	0	27	0	33
		B3501	0.1	0.61	0	35	ND	ND
112-113	_323-332FMCAYPGCNK	B3501	1.3	18.0	0	70	5	45
	_320-334KRPFMCAYPGC	DRB₁0401	0.91	3.48	9	5	5	5
129	_390-398RKFSRSDHL	A0201	1.08	5.81	3	40	ND	ND
131	_398-406LKTHTTRTHT	A0201	1.56	14.0	0	38	ND	ND
141	_436-445NMHQRNHTKL	A0201	1.78	6.69	2	40	0	0
		B4001	2.1	7.71	0	31	3	72
		A2402	0.61	2.79	19	47	0	0

15-mer no. containing the dominant epitope	Sequence identified	Presenting HLA allele	IFNγ response of T cells, % IFNγ⁺ cells		Cytotoxic CTL response, % (at a 50:1 effector: stimulator ratio) vs:
15-mer no. containing the dominant epitope	Sequence identified	Presenting HLA allele	No WT-1 peptide	WT-1 peptide loaded	WT-1⁻ autologous APCs	WT-1 peptide–loaded autologous APCs	WT-1⁻ leukemia cells	WT-1⁺ leukemia cells*
1	_{(−125)-(−117)}RQRPHPGAL	B0702	0.9	11.3	0	27	1	67
2	_{(−119)-(−111)}GALRNPTAC	B0702	0.5	14.0	0	30	1	60
4	_{(−110)-(−102)}PLPHFPPSL	A0201	0.98	5.75	0	30	2	22
5	_{(−107)-(−99)}HFPPSLPPT	A3101	0.73	4.82	0	42	ND	ND
7	_{(−99)-(−91)}THSPTHPPR	B4001	1.5	12.8	0	45	3	65
		A0201	0.4	5	2	50	0	38
13	_(−75)-(67)AILDFLLLQ	A0201	0.61	5.07	0	18	3	19
20	_(−47)-(39)PGCLQQPEQ	A0201	0.2	3.67	6	54	5	19
		B4701	0.5	4.6	6	54	ND	ND
	_{(−47)-(−37)}PGCLQQPEQQG	DRB₁0101	0.33	3.1	6	54	ND	ND
24-25	_{(−27)-(−19)}KLGAAEASA	A0201	1.05	4.48	3	41	10	37
29-30	_(−8)-(1)ASGSEPQQM	B3501	0.07	1.0	5	73	5	39
33	_6-15RDLNALLPAV	A0201	1.1	11.0	2	51	0	9
		B5701	0.19	1.24	3	44	ND	ND
37	_22-31GGCALPVSGA	A0201	0.07	0.9	8	32	3	47
39	_30-38GAAQWAPVL	B3901	0.1	1.3	2	31	ND	ND
41	_38-46LDFAPPGAS	A0201	0.2	4.18	0	73	0	40
	_38-48LDFAPPGASAY	DRB₁0402	0.2	1.41	0	73	0	40
43	_46-54SAYGSLGGP	A0201	1.2	6.46	2	51	0	0
		B4001	1.09	6.84	2	41	3	68
46	_58-66PAPPPPPPP	A0201	1.15	6.69	2	40	0	0
58	_106-114ACRYGPFGP	B4402	0.92	5.65	8	46	ND	ND
62	_122-130SGQARMFPN	B3503	0.78	2.0	0	84	ND	ND
		C0401	0.78	2.0	0	84	ND	ND
62-63	_126-134RMFPNAPYL†	A0201	0.52	2.17	3	41	2	25
65-66	_135-143PSCLESQPA	B3501	0.07	0.61	0	35	ND	ND
68	_146-154NQGYSTVTF	A0101	0.92	4.0	2	19	ND	ND
73	_166-174HHAAQFPNH	B3801	0.81	3.14	0	26	ND	ND
74-75	_174-182HSFKHEDPM	B3501	1.3	18.0	0	50	5	45
82	_202-210CHTPTDSCT	B4402	1.02	3.77	8	37	ND	ND
83-84	_209-217CTGSQALLL	A0101	0.03	0.29	0	21	3	33
83	_206-214TDSCTGSQA	B3802	0.71	4.02	0	88	ND	ND
		B4402	1.01	4.2	1	36	1	56
86	_218-226RTPYSSDNL	B3503	0.84	3.0	0	84	4	48
		C0401	0.84	3.0	0	84	4	48
87	_225-233NLYQMTSQLE	A0201	0.13	0.9	3	87	0	0
91	_238-246WNQMNLGAT	A0201	1.34	8.0	0	18	1	19
		C1701	2.1	12.0	0	10	1	16
		A0101	2.1	7.31	0	26	ND	ND
		B3508	1.23	5.0	0	18	4	19
91-92	_239-248NQMNLGATL	A2402	0.02	0.14	4	9	1	17
91	_238-248WNQMNLGATLK	DRB₁1104	0.59	6.0	0	8	0	0
	_235-249CMTWNQMNLGATLKG	DRB₁0402	0.07	0.53	4	16	1	17
92	_242-250NLGATLKGV	A0101	0.32	1.83	2	19	ND	ND
		A0201	0.06	0.75	1	18	2	19
92-93	_243-252LGATLKGVAA	A0203	0.54	2.1	0	35	ND	ND
93	_246-253TLKGVAAGS	A6901	0.09	1.85	4	80	ND	ND
99-100	_269-278GYESDNHTT	A0101	0.12	2.43	0	27	0	33
		B3501	0.1	0.61	0	35	ND	ND
112-113	_323-332FMCAYPGCNK	B3501	1.3	18.0	0	70	5	45
	_320-334KRPFMCAYPGC	DRB₁0401	0.91	3.48	9	5	5	5
129	_390-398RKFSRSDHL	A0201	1.08	5.81	3	40	ND	ND
131	_398-406LKTHTTRTHT	A0201	1.56	14.0	0	38	ND	ND
141	_436-445NMHQRNHTKL	A0201	1.78	6.69	2	40	0	0
		B4001	2.1	7.71	0	31	3	72
		A2402	0.61	2.79	19	47	0	0

Epitopes are identified by position on the WT-1 protein. T cells are characterized as to their production of IFNγ and their cytotoxic activity against peptide loaded APCs and unloaded WT-1⁺ and WT-1⁻ leukemic cells.

ND indicates not determined.

Leukemia targets were derived from either immortalized leukemia cell lines or primary leukemia cells obtained from patients with WT-1⁺ leukemia.

†

The epitope previously predicted by the computer algorithm or described in the literature.

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