Peripheral blood analysis of transplanted mice at the time of death
| . | No. mice (n) . | Phenotype . | WBC count, 103/μL . | Hemoglobin content, g/dL . | Platelets, 103/μL . | Differentiated myeloid cells (%)* . | Blasts, % . | GFP+ (%) . |
|---|---|---|---|---|---|---|---|---|
| Control | 7 | None | 6.9 (4.6-11.2) | 16 (11.4-17.5) | 499 (335-1203) | 20 (14-31) | 0 | 5 (0.9-6.1) |
| SOCS1 | 10 | None | 6.2 (4.5-16.6) | 14.9 (12.9-16.9) | 256 (131-2612) | 26 (17-33) | 0 | 0.7 (0-2.2) |
| FLT3-ITD | 18 | 12 MPD | 108 (31.4-190.0) | 13.1 (9.6-14.3) | 695 (464-858) | 74 (61-94) | 5 (0-9) | 92 (9.2-96.0) |
| 6 ALL | 90.2 (8.2-146.0) | 13.6 (11.0-16.6) | 497.5 (282-760) | 24 (11-33) | 76 (51-94) | 88.9 (39.3-93.0) | ||
| SOCS1-T2A-FLT3-ITD | 22 | 11 MPD | 51.7 (11.1-80.6) | 13.7 (10.4-17.3) | 421 (227-539) | 71 (58-95) | 6 (0-15) | 66.0 (61.2-91.0) |
| 11 ALL | 56.5 (3.4-133.7) | 14.2 (11.0-17.0) | 422 (312-627) | 23 (14-38) | 81 (22-95) | 88.0 (21.6-97.6) |
| . | No. mice (n) . | Phenotype . | WBC count, 103/μL . | Hemoglobin content, g/dL . | Platelets, 103/μL . | Differentiated myeloid cells (%)* . | Blasts, % . | GFP+ (%) . |
|---|---|---|---|---|---|---|---|---|
| Control | 7 | None | 6.9 (4.6-11.2) | 16 (11.4-17.5) | 499 (335-1203) | 20 (14-31) | 0 | 5 (0.9-6.1) |
| SOCS1 | 10 | None | 6.2 (4.5-16.6) | 14.9 (12.9-16.9) | 256 (131-2612) | 26 (17-33) | 0 | 0.7 (0-2.2) |
| FLT3-ITD | 18 | 12 MPD | 108 (31.4-190.0) | 13.1 (9.6-14.3) | 695 (464-858) | 74 (61-94) | 5 (0-9) | 92 (9.2-96.0) |
| 6 ALL | 90.2 (8.2-146.0) | 13.6 (11.0-16.6) | 497.5 (282-760) | 24 (11-33) | 76 (51-94) | 88.9 (39.3-93.0) | ||
| SOCS1-T2A-FLT3-ITD | 22 | 11 MPD | 51.7 (11.1-80.6) | 13.7 (10.4-17.3) | 421 (227-539) | 71 (58-95) | 6 (0-15) | 66.0 (61.2-91.0) |
| 11 ALL | 56.5 (3.4-133.7) | 14.2 (11.0-17.0) | 422 (312-627) | 23 (14-38) | 81 (22-95) | 88.0 (21.6-97.6) |
Mice transplanted with bone marrow expressing FLT3-ITD or SOCS1-T2A-FLT3-ITD were sacrificed when moribund, whereas mice transplanted with bone marrow expressing control GFP or SOCS1 were sacrificed at the end of the experiment at day 120. Peripheral blood analysis included WBC count, hemoglobin content, platelets, percentage of differentiated myeloid cells and blasts quantified by microscopic quantification of blood smears, and the percentage of GFP expression analyzed by FACS. Values are presented as median (range).
Includes neutrophils and macrophages.