Table 1 Equilibrium dissociation... | American Society of Hematology

Table 1

Equilibrium dissociation constants for the binding of unfractionated heparin to antithrombin

	K_D, nM
Plasma
α-WT	43.9 ± 0.4
β-WT	33.2 ± 1.6
α-L99F	107.9 ± 0.3
β-L99F	53.9 ± 0.2
Recombinant
α-WT	48.9 ± 2.5
β-WT	37.6 ± 2.2
α-P41L	87.1 ± 1.9
β-P41L	39.7 ± 2.1
α-R47C	91.0 ± 1.3
β-R47C	57.8 ± 1.7

	K_D, nM
Plasma
α-WT	43.9 ± 0.4
β-WT	33.2 ± 1.6
α-L99F	107.9 ± 0.3
β-L99F	53.9 ± 0.2
Recombinant
α-WT	48.9 ± 2.5
β-WT	37.6 ± 2.2
α-P41L	87.1 ± 1.9
β-P41L	39.7 ± 2.1
α-R47C	91.0 ± 1.3
β-R47C	57.8 ± 1.7

K_D were measured by global fitting of fluorescence titrations with unfractionated heparin as described.¹⁴

K_D indicates dissociation constant; and WT, wild type.