Histological findings, microvessel density, and vascular endothelial growth factor (VEGF) expression in bone marrow biopsy of 23 patients with Waldenström macroglobulinemia
| Unique patient no. . | VWF:Ag, U/dL . | VWFpp/ VWF:Ag ratio . | Pathologic pattern . | Microvessel density . | VEGF expression . | ||
|---|---|---|---|---|---|---|---|
| Lymphoplasmacytic cells . | Mast cells . | Endothelial cells . | |||||
| 520 | 48 (AVWS) | 6.85 | Diffuse | 2 | 0 | − | − |
| 333 | 47 (AVWS) | 2.06 | Nodular | 2 | 1 | + | − |
| 296 | 64 (AVWS) | 1.84 | Nodular paratrabecular | 1 | 1 | − | − |
| 308 | 69 | NA | Diffuse | 2 | 0 | − | + |
| 357 | 74 | NA | Interstitial paratrabecular | 1 | 0 | − | − |
| 526 | 97 | NA | Nodular | 1 | 0 | + | − |
| 529 | 71 | NA | Diffuse | 2 | 1 | − | − |
| 311 | 107 | 0.76 | Diffuse | 2 | 3 | − | − |
| 321 | 111 | 1.31 | Diffuse | 1 | 0 | − | − |
| 482 | 137 | NA | Interstitial paratrabecular | 1 | 3 | − | + |
| 319 | 150 | NA | Paratrabecular | 1 | 0 | − | − |
| 524 | 199 | NA | Interstitial | 1* | 2 | − | + |
| 501 | 197 | 0.89 | Diffuse | 2 | 3 | − | +/− (localized) |
| 505 | 213 | NA | Diffuse | 2 | 1 | − | − |
| 327 | 150 | 0.67 | Diffuse paratrabecular | 3 | NA* | − | − |
| 368 | 158 | NA | Diffuse | 3 | 1 | + | - |
| 401 | 246 | 1.07 | Interstitial paratrabecular | NA | 2 | ++ | NA |
| 523 | 271 | NA | Diffuse | 2 | 3 | − | + |
| 318 | 322 | NA | Diffuse | 2 | 0 | + | − |
| 307 | 364 | NA | Interstitial paratrabecular | 3 | 0 | + | − |
| 440 | 552 | NA | Diffuse | 3 | 1 | ++ | − |
| 531 | 565 | NA | Diffuse | 3 | 3 | + | +/− (localized) |
| 329 | 804 | NA | Diffuse | 3 | 0 | − | − |
| Unique patient no. . | VWF:Ag, U/dL . | VWFpp/ VWF:Ag ratio . | Pathologic pattern . | Microvessel density . | VEGF expression . | ||
|---|---|---|---|---|---|---|---|
| Lymphoplasmacytic cells . | Mast cells . | Endothelial cells . | |||||
| 520 | 48 (AVWS) | 6.85 | Diffuse | 2 | 0 | − | − |
| 333 | 47 (AVWS) | 2.06 | Nodular | 2 | 1 | + | − |
| 296 | 64 (AVWS) | 1.84 | Nodular paratrabecular | 1 | 1 | − | − |
| 308 | 69 | NA | Diffuse | 2 | 0 | − | + |
| 357 | 74 | NA | Interstitial paratrabecular | 1 | 0 | − | − |
| 526 | 97 | NA | Nodular | 1 | 0 | + | − |
| 529 | 71 | NA | Diffuse | 2 | 1 | − | − |
| 311 | 107 | 0.76 | Diffuse | 2 | 3 | − | − |
| 321 | 111 | 1.31 | Diffuse | 1 | 0 | − | − |
| 482 | 137 | NA | Interstitial paratrabecular | 1 | 3 | − | + |
| 319 | 150 | NA | Paratrabecular | 1 | 0 | − | − |
| 524 | 199 | NA | Interstitial | 1* | 2 | − | + |
| 501 | 197 | 0.89 | Diffuse | 2 | 3 | − | +/− (localized) |
| 505 | 213 | NA | Diffuse | 2 | 1 | − | − |
| 327 | 150 | 0.67 | Diffuse paratrabecular | 3 | NA* | − | − |
| 368 | 158 | NA | Diffuse | 3 | 1 | + | - |
| 401 | 246 | 1.07 | Interstitial paratrabecular | NA | 2 | ++ | NA |
| 523 | 271 | NA | Diffuse | 2 | 3 | − | + |
| 318 | 322 | NA | Diffuse | 2 | 0 | + | − |
| 307 | 364 | NA | Interstitial paratrabecular | 3 | 0 | + | − |
| 440 | 552 | NA | Diffuse | 3 | 1 | ++ | − |
| 531 | 565 | NA | Diffuse | 3 | 3 | + | +/− (localized) |
| 329 | 804 | NA | Diffuse | 3 | 0 | − | − |
VWF:Ag indicates von Willebrand factor antigen; VWFpp, VWF propeptide; AVWS, acquired von Willebrand syndrome; and NA, not available.
Grades for semiquantitative assessment of immunostainings for microvessel density are as follows: 0, similar vascularity in comparison with normal control; 1, slightly increased; 2, moderately increased; and 3, markedly increased. Grades for VEGF on lymphoplasmacytic cells are as follows: 0, no significant staining (< 10% of cells); 1, staining in 10%-50% of cells; 2, moderate staining in more than 50% of cells; and 3, strong staining in more than 50% of cells. Cytoplasmic staining of VEGF in mast cells is as follows: −, absent; +, weak; and ++, moderate. Cytoplasmic staining of endothelial cells is as follows: −, absent; and +, present.
Material was dissociated and/or hemorrhagic.