Intracellular bortezomib concentrations in RPMI 8226.wt and 8226.BR
| Cell line . | Time after treatment, h . | Bortezomib, ng/mL . | Bortezomib, ng/1 × 106 cells . |
|---|---|---|---|
| 8226.wt | 0 | n.d. | n.d. |
| 1 | 0.13 | 0.16 | |
| 3 | 0.32 | 0.40 | |
| 6 | 0.38 | 0.48 | |
| 24 | 0.19 | 0.24 | |
| 48 | n.d. | n.d. | |
| 8226.BR | 0 | n.d. | n.d. |
| 1 | 0.52 | 0.65 | |
| 3 | 2.46 | 3.08 | |
| 6 | 3.45 | 4.31 | |
| 24 | 3.43 | 4.29 | |
| 48 | 2.01 | 2.51 |
| Cell line . | Time after treatment, h . | Bortezomib, ng/mL . | Bortezomib, ng/1 × 106 cells . |
|---|---|---|---|
| 8226.wt | 0 | n.d. | n.d. |
| 1 | 0.13 | 0.16 | |
| 3 | 0.32 | 0.40 | |
| 6 | 0.38 | 0.48 | |
| 24 | 0.19 | 0.24 | |
| 48 | n.d. | n.d. | |
| 8226.BR | 0 | n.d. | n.d. |
| 1 | 0.52 | 0.65 | |
| 3 | 2.46 | 3.08 | |
| 6 | 3.45 | 4.31 | |
| 24 | 3.43 | 4.29 | |
| 48 | 2.01 | 2.51 |
wt indicates wild-type; BR, bortezomib-resistant cells; and n.d., not detected (less than limit of detection).