Summary of clinical, pathological, immunohistochemical, and cytogenetic features of patients enrolled in the study
| EBI ID . | Age, y . | Sex . | Diagnosis . | Organ . | Type of sample . | CD3 . | CD2 . | CD5 . | CD7 . | CD4 . | CD8 . | βF1 . | δTCR . | CD56 . | TiA1 . | GzmB . | EBV* . | T clone . | Cytogenetics . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HSTL_02 | 41 | F | HSTLγδ | Spleen | Tissue | + | + | - | - | - | - | - | +† | + | + | - | - | + | iso7q (+) |
| HSTL_03 | 73 | F | HSTLγδ | Spleen | Tissue | + | + | - | - | - | + | - | + | + | + | + | - | + | iso7q (−) |
| HSTL_04 | 38 | M | HSTLγδ | Spleen | Tissue | + | ND | - | - | - | - | + | +‡ | - | + | - | - | + | iso7q (+) trisomy 8 |
| HSTL_05 | 56 | M | HSTL αβ | Spleen | Tissue | + | ND | - | ND | - | - | + | - | - | - | - | - | + | iso7q (+) |
| HSTL_06 | 53 | F | HSTL αβ | Spleen | Tissue | + | - | - | + | - | - | + | - | + | + | - | - | + | iso7q (−) |
| HSTL_08 | 24 | F | HSTLγδ | Blood | Cell | + | ND | - | + | - | - | - | + | + | + | ND | ND | + | |
| HSTL_09 | 36 | M | HSTLγδ | Blood | Cell (purified CD56+, 77%) | + | + | - | + | - | - | - | + | + | + | - | ND | + | iso7q (+) |
| HSTL_01 | 86 | F | HSTLγδ | Spleen | Cell (purified CD56+, 84%) | + | + | -/+ | ND | - | - | - | + | + | + | - | - | + | iso7q (−) |
| HSTL_10 | 67 | M | HSTLγδ | Spleen | Tissue | + | + | - | + | - | - | - | +‡ | - | + | - | - | + | Iso7q (−) |
| HSTL_07 DERL2 | 30 | M | HSTLγδ | Blood | Cell line | + | + | - | + | - | + | + | + | - | Iso7q (+) |
| EBI ID . | Age, y . | Sex . | Diagnosis . | Organ . | Type of sample . | CD3 . | CD2 . | CD5 . | CD7 . | CD4 . | CD8 . | βF1 . | δTCR . | CD56 . | TiA1 . | GzmB . | EBV* . | T clone . | Cytogenetics . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HSTL_02 | 41 | F | HSTLγδ | Spleen | Tissue | + | + | - | - | - | - | - | +† | + | + | - | - | + | iso7q (+) |
| HSTL_03 | 73 | F | HSTLγδ | Spleen | Tissue | + | + | - | - | - | + | - | + | + | + | + | - | + | iso7q (−) |
| HSTL_04 | 38 | M | HSTLγδ | Spleen | Tissue | + | ND | - | - | - | - | + | +‡ | - | + | - | - | + | iso7q (+) trisomy 8 |
| HSTL_05 | 56 | M | HSTL αβ | Spleen | Tissue | + | ND | - | ND | - | - | + | - | - | - | - | - | + | iso7q (+) |
| HSTL_06 | 53 | F | HSTL αβ | Spleen | Tissue | + | - | - | + | - | - | + | - | + | + | - | - | + | iso7q (−) |
| HSTL_08 | 24 | F | HSTLγδ | Blood | Cell | + | ND | - | + | - | - | - | + | + | + | ND | ND | + | |
| HSTL_09 | 36 | M | HSTLγδ | Blood | Cell (purified CD56+, 77%) | + | + | - | + | - | - | - | + | + | + | - | ND | + | iso7q (+) |
| HSTL_01 | 86 | F | HSTLγδ | Spleen | Cell (purified CD56+, 84%) | + | + | -/+ | ND | - | - | - | + | + | + | - | - | + | iso7q (−) |
| HSTL_10 | 67 | M | HSTLγδ | Spleen | Tissue | + | + | - | + | - | - | - | +‡ | - | + | - | - | + | Iso7q (−) |
| HSTL_07 DERL2 | 30 | M | HSTLγδ | Blood | Cell line | + | + | - | + | - | + | + | + | - | Iso7q (+) |
EBV investigation by in situ hybridization with EBER probes and/or immunohistochemistry with LMP-1 Ab.
HSTL_02 with a γδ HSTL phenotype at diagnosis disclosed a TCR-silent phenotype (βF1−δTCR1−) at the time of analysis.
δ chain rearrangement determination using a GC-clamp multiplex PCR–δ-DGGE procedure.
ND indicates not determined.