Leukocyte rolling and adhesion in inflamed mesenteric venules
| Parameter* . | PBS . | Thioglycollate + control Ab . | Thioglycollate + anti-VWF Ab . |
|---|---|---|---|
| Leukocyte rolling (n/30 s) | 6.1 × 6.0 | 30.9 × 5.0 | 29.2 × 3.0 |
| Leukocyte adhesion (n/104 μm2) | 0.2 × 0.1 | 3.3 × 0.4 | 3.1 × 0.2 |
| Vessel diameter (μm) | 23.6 × 1.8 | 29.5 × 0.4 | 28.1 × 0.4 |
| Parameter* . | PBS . | Thioglycollate + control Ab . | Thioglycollate + anti-VWF Ab . |
|---|---|---|---|
| Leukocyte rolling (n/30 s) | 6.1 × 6.0 | 30.9 × 5.0 | 29.2 × 3.0 |
| Leukocyte adhesion (n/104 μm2) | 0.2 × 0.1 | 3.3 × 0.4 | 3.1 × 0.2 |
| Vessel diameter (μm) | 23.6 × 1.8 | 29.5 × 0.4 | 28.1 × 0.4 |
Intravital microscopic assessment of leukocyte-endothelial interactions. Two hours after thioglycollate-mediated induction of peritonitis and injection of VWF- or anti-ESAM negative control antibodies intravital microscopy was performed on 7-15 single unbranched postcapillary mesenteric venules per animal (5 mice per group). Control animals received PBS instead of thioglycollate. Rolling leukocytes were quantified for 30 seconds. The number of adherent leukocytes was determined for each venule segment (100 μm) and was expressed per 104 μm2 of venule surface area. Data from 1 representative analysis of 3 are shown.