Checkerboard analysis of ATP-induced migration
| ATP, μM . | 0μM ATP . | 0.1μM ATP . | 1μM ATP . | 10μM ATP . |
|---|---|---|---|---|
| 0 | — | 1.1 | 2.2 | 6.1 |
| 0.1 | 1.0 | 1.0 | 2.1 | 5.0 |
| 1 | 7.7 | 1.5 | 2.2 | 5.3 |
| 10 | 3.5 | 3.2 | 2.6 | 5.9 |
| ATP, μM . | 0μM ATP . | 0.1μM ATP . | 1μM ATP . | 10μM ATP . |
|---|---|---|---|---|
| 0 | — | 1.1 | 2.2 | 6.1 |
| 0.1 | 1.0 | 1.0 | 2.1 | 5.0 |
| 1 | 7.7 | 1.5 | 2.2 | 5.3 |
| 10 | 3.5 | 3.2 | 2.6 | 5.9 |
Migration assay was performed as indicated in “Migration assays,” adding ATP to the top and bottom chambers of the transwell (checkerboard analysis) as indicated. Data represent the migration index calculated as the ratio between the number of cells migrated in each condition and the number of cells migrated in the absence of ATP. Results are shown from 1 representative experiment of 3 performed.
— indicates not applicable.