Murine BM transplantation experiments
| . | Virus titer, CFU/mL . | BM-infection efficacy % . | n = mice . | EGFP+ cells transplanted . | Cells transplanted . | d30 WBC, G/L . | d30 HCT, % . | d30 Hb, g/dL . | d30 Retic., ‰ . | d30 Platelets, G/L . | d60 WBC, G/L . | d60 HCT, % . | d60 Hb, g/dL . | d60 Retic., ‰ . | d60 Platelets, G/L . | Spleen weight, mg . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1st TX | ||||||||||||||||
| Mock | 5 × 105 | 23 | 2 | 50 000 | 215 000 | 10 ± 4 | 42 ± 2 | 16 ± 1.5 | 32 ± 2 | 1208 ± 270 | 13 ± 1.2 | 44 ± 2 | 19 ± 2 | 37 ± 2 | 938 ± 370 | 83.5 ± 23 |
| V617F | 4.3 × 105 | 17 | 4 | 50 000 | 295 000 | 20 ± 6 | 67 ± 3 | 27 ± 2.1 | 55 ± 10 | 1148 ± 366 | 38.5 ± 4 | 74 ± 2 | 32 ± 2 | 67.5 ± 10 | 871 ± 200 | 600 ± 40 |
| V617F | 4 × 105 | 16.5 | 3 | 50 000 | 300 000 | 9.9 ± 4 | 43 ± 2 | 18 ± 1.2 | 28 ± 6 | 816 ± 429 | 13 ± 2 | 42 ± 5 | 17.3 ± 1 | 37.5 ± 10 | 1438 ± 507 | 98 ± 45 |
| mSH2 | ||||||||||||||||
| 2nd TX | ||||||||||||||||
| Mock | 1 × 106 | 50 | 2 | 50 000 | 100 000 | 9.75 ± 1.5 | 56.5 ± 0.7 | 16.9 ± 0.7 | 45 ± 1.5 | 1068 ± 85 | 12.3 ± 3 | 59.2 ± 7 | 16.8 ± 2 | 35 | 780 ± 133 | 187 |
| V617F | 8 × 105 | 38.9 | 4 | 50 000 | 130 000 | 84.7 ± 16 | 90.5 ± 3.8 | 24 ± 1.2 | 99 ± 12.5 | 733 ± 110 | 157.5 ± 15 | 87.5 ± 11 | 25.3 ± 3.7 | 84.5 ± 8.3 | 468 ± 184 | 800 ± 5 |
| V617F | 7.8 × 105 | 38.8 | 3 | 50 000 | 130 000 | 10 ± 0.5 | 60.3 ± 0.5 | 18.3 ± 0.8 | 38.6 ± 3 | 806 ± 339 | 14.9 ± 1 | 63.3 ± 1.5 | 18 | 39.6 ± 3.2 | 994 ± 45 | 201 ± 10 |
| mSH2 | ||||||||||||||||
| 3rd TX | ||||||||||||||||
| V617F | 4 × 105 | 19 | 4 | 50 000 | 270 000 | 24.8 ± 2.5 | 89 ± 2 | 22.3 ± 1.4 | ND | 600 ± 89 | 41.6 ± 8 | 83.5 ± 13 | 24.7 ± 4 | ND | 604 ± 155 | ND |
| V617F | 4.5 × 105 | 18.3 | 4 | 50 000 | 270 000 | 9.6 ± 2 | 56 ± 1.5 | 16 ± 1.3 | ND | 815 ± 121 | 10.9 ± 2.6 | 54 ± 2.5 | 16 ± 1.3 | ND | 1145 ± 125 | ND |
| mSH2 |
| . | Virus titer, CFU/mL . | BM-infection efficacy % . | n = mice . | EGFP+ cells transplanted . | Cells transplanted . | d30 WBC, G/L . | d30 HCT, % . | d30 Hb, g/dL . | d30 Retic., ‰ . | d30 Platelets, G/L . | d60 WBC, G/L . | d60 HCT, % . | d60 Hb, g/dL . | d60 Retic., ‰ . | d60 Platelets, G/L . | Spleen weight, mg . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1st TX | ||||||||||||||||
| Mock | 5 × 105 | 23 | 2 | 50 000 | 215 000 | 10 ± 4 | 42 ± 2 | 16 ± 1.5 | 32 ± 2 | 1208 ± 270 | 13 ± 1.2 | 44 ± 2 | 19 ± 2 | 37 ± 2 | 938 ± 370 | 83.5 ± 23 |
| V617F | 4.3 × 105 | 17 | 4 | 50 000 | 295 000 | 20 ± 6 | 67 ± 3 | 27 ± 2.1 | 55 ± 10 | 1148 ± 366 | 38.5 ± 4 | 74 ± 2 | 32 ± 2 | 67.5 ± 10 | 871 ± 200 | 600 ± 40 |
| V617F | 4 × 105 | 16.5 | 3 | 50 000 | 300 000 | 9.9 ± 4 | 43 ± 2 | 18 ± 1.2 | 28 ± 6 | 816 ± 429 | 13 ± 2 | 42 ± 5 | 17.3 ± 1 | 37.5 ± 10 | 1438 ± 507 | 98 ± 45 |
| mSH2 | ||||||||||||||||
| 2nd TX | ||||||||||||||||
| Mock | 1 × 106 | 50 | 2 | 50 000 | 100 000 | 9.75 ± 1.5 | 56.5 ± 0.7 | 16.9 ± 0.7 | 45 ± 1.5 | 1068 ± 85 | 12.3 ± 3 | 59.2 ± 7 | 16.8 ± 2 | 35 | 780 ± 133 | 187 |
| V617F | 8 × 105 | 38.9 | 4 | 50 000 | 130 000 | 84.7 ± 16 | 90.5 ± 3.8 | 24 ± 1.2 | 99 ± 12.5 | 733 ± 110 | 157.5 ± 15 | 87.5 ± 11 | 25.3 ± 3.7 | 84.5 ± 8.3 | 468 ± 184 | 800 ± 5 |
| V617F | 7.8 × 105 | 38.8 | 3 | 50 000 | 130 000 | 10 ± 0.5 | 60.3 ± 0.5 | 18.3 ± 0.8 | 38.6 ± 3 | 806 ± 339 | 14.9 ± 1 | 63.3 ± 1.5 | 18 | 39.6 ± 3.2 | 994 ± 45 | 201 ± 10 |
| mSH2 | ||||||||||||||||
| 3rd TX | ||||||||||||||||
| V617F | 4 × 105 | 19 | 4 | 50 000 | 270 000 | 24.8 ± 2.5 | 89 ± 2 | 22.3 ± 1.4 | ND | 600 ± 89 | 41.6 ± 8 | 83.5 ± 13 | 24.7 ± 4 | ND | 604 ± 155 | ND |
| V617F | 4.5 × 105 | 18.3 | 4 | 50 000 | 270 000 | 9.6 ± 2 | 56 ± 1.5 | 16 ± 1.3 | ND | 815 ± 121 | 10.9 ± 2.6 | 54 ± 2.5 | 16 ± 1.3 | ND | 1145 ± 125 | ND |
| mSH2 |
Details of 3 independent transplantation experiments are shown including viral titers used, infection efficiencies, numbers of transplanted cells, as well as blood counts of transplanted animals over time, and spleen weights. Retroviral titers were determined by transduction of 5 × 104 NIH3T3 cells with serial dilutions of retrovirus. Forty-eight hours after transduction, the percentage of infected cells was determined by flow cytometric analysis of eGFP expression. The titer (CFU/mL) was calculated by multiplication of the total number of eGFP-positive cells with the dilution factor of the retroviral supernatant.
TX indicates transplantation; CFU, colony-forming units; WBC, white blood cell count; HCT, hematocrit; Hb, hemoglobin; Retic., reticulocytes; d, day; and ND, not determined.