Table 1 Specific activity of PRC2... | American Society of Hematology

Table 1

Specific activity of PRC2 complexes

PRC2 complex containing	Enzyme activity* on various histone H3 (21-44) K27 substrates with indicated methylation states
PRC2 complex containing	Unmethylated	Monomethylated	Dimethylated
EZH2^WT	10.28 (r² = 0.99)	9.55 (r² = 1.00)	4.90 (r² = 0.95)
EZH2^Y641N	−0.40 (r² = 0.98)	0.59 (r² = 0.04)	9.01 (r² = 0.92)
EZH2^Y641F	0.45 (r² = 0.37)	1.09 (r² = 0.61)	9.86 (r² = 0.99)

PRC2 complex containing	Enzyme activity* on various histone H3 (21-44) K27 substrates with indicated methylation states
PRC2 complex containing	Unmethylated	Monomethylated	Dimethylated
EZH2^WT	10.28 (r² = 0.99)	9.55 (r² = 1.00)	4.90 (r² = 0.95)
EZH2^Y641N	−0.40 (r² = 0.98)	0.59 (r² = 0.04)	9.01 (r² = 0.92)
EZH2^Y641F	0.45 (r² = 0.37)	1.09 (r² = 0.61)	9.86 (r² = 0.99)

The specific activity (units/ng per minute) of PRC2 complexes containing EZH2^WT, EZH2^Y641N, or EZH2^Y641F was calculated by linear regression from the graphs in Figure 4B.