Hematopoietic reconstitution capacity of human EL 34DP cells and 34HCs in primary NOD/SCID mice
| Age, weeks of gestation . | Sorted cells . | No. of injected cells . | Engrafted mice* . |
|---|---|---|---|
| Total mice . | |||
| 8.4 | 34DP | 5000 | 2/2 |
| 34HC | 5000 | 0/2 | |
| 50 000 | 0/2 | ||
| 7.8 | 34DP | 5000 | 2/2 |
| 34HC | 5000 | 0/2 | |
| 50 000 | 0/2 | ||
| 7 | 34DP | 5000 | 2/2 |
| 34HC | 5000 | 0/2 | |
| 50 000 | 1/2 |
| Age, weeks of gestation . | Sorted cells . | No. of injected cells . | Engrafted mice* . |
|---|---|---|---|
| Total mice . | |||
| 8.4 | 34DP | 5000 | 2/2 |
| 34HC | 5000 | 0/2 | |
| 50 000 | 0/2 | ||
| 7.8 | 34DP | 5000 | 2/2 |
| 34HC | 5000 | 0/2 | |
| 50 000 | 0/2 | ||
| 7 | 34DP | 5000 | 2/2 |
| 34HC | 5000 | 0/2 | |
| 50 000 | 1/2 |
Engraftment is based on flow cytometric analysis of BM cells from recipient mice harvested at 4 months after transplantation and is defined as more than 1% human CD45+ cells.