Both CCR6+ central memory and effector memory CD4+ T cells produced greater amounts of TNF-α than CCR6− T cells
| . | Central memory CD4 T cells . | Effector memory CD4 T cells . | ||
|---|---|---|---|---|
| CCR6− . | CCR6+ . | CCR6− . | CCR6+ . | |
| Anti-CD2/CD3/CD28 | 23 | 43 | 28 | 46 |
| SEB | 0.15 | 1.5 | 2 | 7 |
| . | Central memory CD4 T cells . | Effector memory CD4 T cells . | ||
|---|---|---|---|---|
| CCR6− . | CCR6+ . | CCR6− . | CCR6+ . | |
| Anti-CD2/CD3/CD28 | 23 | 43 | 28 | 46 |
| SEB | 0.15 | 1.5 | 2 | 7 |
T-cell memory subsets were sorted according to their expression of CD45RA, CCR7, and CCR6 and stimulated overnight with polyclonal stimulation in the presence of brefeldin A for cytokine production assay. Results are shown as the percentage of TNF-α-secreting CD4+ T cells expressing or not CCR6 and represent 2 independent experiments.