Ectopic PAX5 expression sustains long-term generation of granulocytes
| . | Blasts . | Granulocytes . | Monocytes/macrophages . | Mast cells . | |
|---|---|---|---|---|---|
| Immature . | Mature . | ||||
| Control | 0 (0; 0) | 0.6 (0-2; 1.2) | 30.7 (0-61; 30.5) | 35.7 (9-65; 28) | 33 (2-91; 50.3) |
| PAX5 | 2.7* (2-3; 0.6) | 41* (37-46; 4.6) | 43.6 (40-50; 5.5) | 8.1 (4-11; 3.8) | 4.6 (2-6; 2.3) |
| . | Blasts . | Granulocytes . | Monocytes/macrophages . | Mast cells . | |
|---|---|---|---|---|---|
| Immature . | Mature . | ||||
| Control | 0 (0; 0) | 0.6 (0-2; 1.2) | 30.7 (0-61; 30.5) | 35.7 (9-65; 28) | 33 (2-91; 50.3) |
| PAX5 | 2.7* (2-3; 0.6) | 41* (37-46; 4.6) | 43.6 (40-50; 5.5) | 8.1 (4-11; 3.8) | 4.6 (2-6; 2.3) |
Control or PAX5-transduced LSK cells were cultured with SCF, GM-CSF, IL-3, and G-CSF for 24 days at which time cytospin slides were stained with May-Grünwald-Giemsa (MGG) and evaluated under a light microscope. Data represent mean percentages (range; SD) of blasts, granulocytes (immature and mature), monocytes/macrophages, and mast cells derived from control and PAX5-transduced lines in 3 different experiments.
P < .05.