Characteristics of CLL patients studied
| CLL patient label . | Apoptotic response S-US at 6 h . | IgVH status (%) . | Zap-70 status . | Cytogenetics . |
|---|---|---|---|---|
| Indolent | ||||
| CLL-M4 | 21.2 | M-CLL VH3-23 (93) | Negative | Normal |
| CLL-M5 | 24.8 | M-CLL VH3-22 (87) | Negative | 13q− |
| CLL-M6 | 19.6 | M-CLL VH3-23 (92) | Negative | 13q− |
| CLL-M7 | 28.8 | M-CLL VH4-31 (86) | Negative | 13q− |
| CLL-M3 | 32.5 | M-CLL VH3-48 (91) | Negative | 13q− |
| CLL-M8 | 33.6 | M-CLL VH4-34 (94) | Negative | 13q− |
| CLL-M1 | ND | M-CLL VH4-31 (95) | Negative | 13q− |
| Aggressive | ||||
| CLL-UM1 | 20.5 | UM-CLL VH1-69 (99) | Positive | Add 12 |
| CLL-UM2 | 41.7 | UM-CLL VH1-69 (100) | Positive | t(8;13), add8, 11q− |
| CLL-UM3 | 41.7 | UM-CLL VH1-69 (100) | Positive | Add 12 |
| CLL-UM4 | 53.2 | UM-CLL VH4-34 (100) | Positive | Normal |
| CLL-M2 | 44.7 | M-CLL VH3-48 (97) | Positive | Normal |
| CLL patient label . | Apoptotic response S-US at 6 h . | IgVH status (%) . | Zap-70 status . | Cytogenetics . |
|---|---|---|---|---|
| Indolent | ||||
| CLL-M4 | 21.2 | M-CLL VH3-23 (93) | Negative | Normal |
| CLL-M5 | 24.8 | M-CLL VH3-22 (87) | Negative | 13q− |
| CLL-M6 | 19.6 | M-CLL VH3-23 (92) | Negative | 13q− |
| CLL-M7 | 28.8 | M-CLL VH4-31 (86) | Negative | 13q− |
| CLL-M3 | 32.5 | M-CLL VH3-48 (91) | Negative | 13q− |
| CLL-M8 | 33.6 | M-CLL VH4-34 (94) | Negative | 13q− |
| CLL-M1 | ND | M-CLL VH4-31 (95) | Negative | 13q− |
| Aggressive | ||||
| CLL-UM1 | 20.5 | UM-CLL VH1-69 (99) | Positive | Add 12 |
| CLL-UM2 | 41.7 | UM-CLL VH1-69 (100) | Positive | t(8;13), add8, 11q− |
| CLL-UM3 | 41.7 | UM-CLL VH1-69 (100) | Positive | Add 12 |
| CLL-UM4 | 53.2 | UM-CLL VH4-34 (100) | Positive | Normal |
| CLL-M2 | 44.7 | M-CLL VH3-48 (97) | Positive | Normal |
Indolent: average, 26.8; median, 26.8. Aggressive: average, 40.4; median, 41.7.
Apoptotic response measured by flow cytometry after Annexin V–FITC and propidium iodide double staining, with percentage of BCR-stimulated cells compared to unstimulated cells undergoing Annexin V[e]FITC staining 6 hours after BCR cross-linking. Cytogenetics characteristics were assessed by FISH.
UM indicates IgVH unmutated; M, IgVH mutated; and ND, not determined.