Summary of main findings on the signaling and adhesive properties of Kindlin-3–null LAD-III–derived PB T cells
| Readout . | Kindlin-3 null cells . |
|---|---|
| Surface expression of integrins CCR7 and CD3 | Normal |
| Motility on immobilized CCL21 | Normal |
| Soluble CCL21 induction of the 327C β2 activation epitope | Impaired |
| Soluble CCL21-triggered microvillar collapse | Normal |
| TCR-mediated LAT phosphorylation and spreading | Normal |
| TCR-triggered spreading on ICAM-1 | Impaired |
| TCR-triggered generation of headpiece-activated LFA-1 during spreading on ICAM-1 | In a peripheral ring only |
| TCR-triggered generation of scattered activated LFA-1 focal dots during spreading on ICAM-1 | Impaired |
| TCR-triggered LFA-1–dependent spreading on DCs | Impaired |
| Shear-resistant adhesion to immobilized anti–LFA-1 integrin mAbs | Reduced |
| Generation of 327C-bearing β2 integrins at the leading edge during motility on immobilized CCL21 | Normal |
| Responsiveness of primed (327C-positive) LFA-1 to TCR-triggered stop signals on ICAM-1 in motile T cells | Impaired |
| TCR-triggered firm contacts to DCs | Impaired |
| Readout . | Kindlin-3 null cells . |
|---|---|
| Surface expression of integrins CCR7 and CD3 | Normal |
| Motility on immobilized CCL21 | Normal |
| Soluble CCL21 induction of the 327C β2 activation epitope | Impaired |
| Soluble CCL21-triggered microvillar collapse | Normal |
| TCR-mediated LAT phosphorylation and spreading | Normal |
| TCR-triggered spreading on ICAM-1 | Impaired |
| TCR-triggered generation of headpiece-activated LFA-1 during spreading on ICAM-1 | In a peripheral ring only |
| TCR-triggered generation of scattered activated LFA-1 focal dots during spreading on ICAM-1 | Impaired |
| TCR-triggered LFA-1–dependent spreading on DCs | Impaired |
| Shear-resistant adhesion to immobilized anti–LFA-1 integrin mAbs | Reduced |
| Generation of 327C-bearing β2 integrins at the leading edge during motility on immobilized CCL21 | Normal |
| Responsiveness of primed (327C-positive) LFA-1 to TCR-triggered stop signals on ICAM-1 in motile T cells | Impaired |
| TCR-triggered firm contacts to DCs | Impaired |