Table 2 DMOG and Dex synergize to... | American Society of Hematology

Table 2

DMOG and Dex synergize to prevent BFU-E cell exhaustion and enhance CFU-E regeneration: total colony numbers from 100 BFU-E cells

Dex/DMOG	Day
Dex/DMOG	0	1	2	3	4	5	6	7	8
CFU-E, 1 cluster
Nothing added	3	98	630	1100*	1000	450	50
DMOG	3	64	600	2400	2800*	2700	1000
Dex	3	42	1100	5100	14 000	28 000*	25 000	18 000	6600
DMOG + Dex	3	19	870	8000	27 000	94 000	150 000	190 000*	160 000
Small BFU-E, 5-20 clusters
Nothing added	26	130	240*	200		53
DMOG	26	130	320	440*		100
Dex	26	180	580	830		1000*
DMOG + Dex	26	150	790	2000		3800*
Large BFU-E, > 20 clusters
Nothing added	42*	28	15	12		0
DMOG	42	43*	22	17		0
Dex	42	45	49*	40		0
DMOG + Dex	42	72	120	160*		8

Dex/DMOG	Day
Dex/DMOG	0	1	2	3	4	5	6	7	8
CFU-E, 1 cluster
Nothing added	3	98	630	1100*	1000	450	50
DMOG	3	64	600	2400	2800*	2700	1000
Dex	3	42	1100	5100	14 000	28 000*	25 000	18 000	6600
DMOG + Dex	3	19	870	8000	27 000	94 000	150 000	190 000*	160 000
Small BFU-E, 5-20 clusters
Nothing added	26	130	240*	200		53
DMOG	26	130	320	440*		100
Dex	26	180	580	830		1000*
DMOG + Dex	26	150	790	2000		3800*
Large BFU-E, > 20 clusters
Nothing added	42*	28	15	12		0
DMOG	42	43*	22	17		0
Dex	42	45	49*	40		0
DMOG + Dex	42	72	120	160*		8

CFU-E and BFU-E colony-forming assays were performed at 24-hour intervals in the presence of 100nM Dex. Shown are the number of different types of colonies formed per 100 “BFU-E” cells plated at 0 hours.

Number of erythroblasts or colony-forming cells reached its highest value that day.

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