Table 1 DMOG and Dex synergize to... | American Society of Hematology

Table 1

DMOG and Dex synergize to prevent BFU-E cell exhaustion and enhance CFU-E regeneration: total cell expansion from 100 BFU-E cells

Dex/DMOG	Day
Dex/DMOG	0	1	2	3	4	5	6	7	8	9	10	11
Nothing added	100	400	3100	9210	34 000	50 000	58 000*	49 000	54 000
DMOG	100	420	2600	13 000	43 000	82 000	89 000	120 000*	120 000
Dex	100	390	3000	20 000	120 000	350 000	720 000	1 400 000	2 000 000	2 500 000*	2 500 000	2 000 000
DMOG + Dex	100	410	3700	23 000	130 000	520 000	1 200 000	4 900 000	8 000 000	12 000 000	18 000 000*	12 000 000

Dex/DMOG	Day
Dex/DMOG	0	1	2	3	4	5	6	7	8	9	10	11
Nothing added	100	400	3100	9210	34 000	50 000	58 000*	49 000	54 000
DMOG	100	420	2600	13 000	43 000	82 000	89 000	120 000*	120 000
Dex	100	390	3000	20 000	120 000	350 000	720 000	1 400 000	2 000 000	2 500 000*	2 500 000	2 000 000
DMOG + Dex	100	410	3700	23 000	130 000	520 000	1 200 000	4 900 000	8 000 000	12 000 000	18 000 000*	12 000 000

Sorted BFU-E (CD24 and CD71^10%low) cells were cultured in SFELE medium with or without 100nM Dex and with or without 333μM DMOG (Figure 5B). Although the average of 4 experiments is shown, CFU-E colony-forming assays at days 7 and 8 were performed only twice. Data reported are number of cells formed in culture from 100 BFU-E cells. Results show that a combination of DMOG and Dex reduces differentiation and increases self-renewal of BFU-E cells, which over time results in increased CFU-E colony formation.

Number of erythroblasts reached its highest value that day.

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