IPA in mother's blood and the survival of mother's lymphocytes in patient's blood
| Case . | Mother's PBMCs infused per infusion, ×108/kg . | IPAs in mother's blood* . | Ratio of NIMAs in patient's blood before the infusion* . | Ratio of NIMAs in patient's blood after the infusion* . |
|---|---|---|---|---|
| 1; 4 infusions in total | 2 | SRY+; | Negative | 4 days after the first infusion: 10−3.9; |
| 1.3 | 10−5.15 by quantification | 8 days after the first infusion: none | ||
| 1.0 | ||||
| 1.1 | ||||
| 2; 4 infusions in total | 6.8 | SRY+; | † | † |
| 5.7 | 10−4.12 by quantification | |||
| 3.6 | ||||
| 4 | ||||
| 3; 2 infusions in total | 1.1 | SRY+; | 10−3.13 | 5 days after the first infusion: 10−2.84; |
| 1.0 | † | 13 months after the first infusion: 10−4.92 | ||
| 4 | 3.5 | SRY+; 10−4.67 by quantification | † | † |
| 5 | 1.7 | SRY+; † | 10−2.33 | 4 days after the first infusion:10−2.46; 42 days after the first infusion:10−2.61 |
| Case . | Mother's PBMCs infused per infusion, ×108/kg . | IPAs in mother's blood* . | Ratio of NIMAs in patient's blood before the infusion* . | Ratio of NIMAs in patient's blood after the infusion* . |
|---|---|---|---|---|
| 1; 4 infusions in total | 2 | SRY+; | Negative | 4 days after the first infusion: 10−3.9; |
| 1.3 | 10−5.15 by quantification | 8 days after the first infusion: none | ||
| 1.0 | ||||
| 1.1 | ||||
| 2; 4 infusions in total | 6.8 | SRY+; | † | † |
| 5.7 | 10−4.12 by quantification | |||
| 3.6 | ||||
| 4 | ||||
| 3; 2 infusions in total | 1.1 | SRY+; | 10−3.13 | 5 days after the first infusion: 10−2.84; |
| 1.0 | † | 13 months after the first infusion: 10−4.92 | ||
| 4 | 3.5 | SRY+; 10−4.67 by quantification | † | † |
| 5 | 1.7 | SRY+; † | 10−2.33 | 4 days after the first infusion:10−2.46; 42 days after the first infusion:10−2.61 |
Quantification of microchimerism by specific insertion/deletion polymorphism method.
We used 9 insertion/deletion polymorphisms, trying to identify the polymorphism specific for IPAs or NIMAs, but no such insertion/deletion polymorphism was found. Therefore, quantification of microchimerism could not be conducted.