Extent of colocalization of phospho-ZAP-70 with high-affinity LFA-1 and Talin
| . | Lamellipodal/lamella junction . | Mid cell lamella . |
|---|---|---|
| pZAP-70/24+ LFA-1 | 60.8 ± 17.7 | 62.2 ± 6.8 |
| pZAP-70/talin | 52.4 ± 11.4 | 59.6 ± 10.8 |
| 24+ LFA-1/talin | 74.3 ± 10.4 | 86.2 ± 9.2 |
| . | Lamellipodal/lamella junction . | Mid cell lamella . |
|---|---|---|
| pZAP-70/24+ LFA-1 | 60.8 ± 17.7 | 62.2 ± 6.8 |
| pZAP-70/talin | 52.4 ± 11.4 | 59.6 ± 10.8 |
| 24+ LFA-1/talin | 74.3 ± 10.4 | 86.2 ± 9.2 |
A pixel-by-pixel analysis of the overlap of phospho-ZAP-70 with 24 epitope that detects high-affinity LFA-1 and also with talin. A third comparison is between 24 + LFA-1 and talin. Data are shown as the percentage of overlap in fluorescence ± SD; n = 10 cells analyzed for each set of mAbs.