Distribution of L-, H-, and S-subunits in liver, heart, and serum ferritin
| . | Liver (%) . | Heart (%) . | Serum (%) . |
|---|---|---|---|
| Coomassie | |||
| L-subunit | 74.2 ± 2.2 | 65.8 ± 9.4 | 41.2 ± 6.2 |
| H-subunit | 10 ± 3.3 | 18.8 ± 7.1 | 5.3 ± 2.9 |
| S-subunit | 15.8 ± 2.9 | 15.4 ± 3.7 | 53.5 ± 7.7 |
| Western-blot | |||
| L-subunit | 68.61 ± 0.34 | 65.60 ± 2.05 | 47.03 ± 2.05 |
| H-subunit | 10* | 18.16 ± 0.95 | 3.26 ± 1.94 |
| S-subunit | 21.39 ± 0.34 | 16.24 ± 1.44 | 49.71 ± 2.09 |
| . | Liver (%) . | Heart (%) . | Serum (%) . |
|---|---|---|---|
| Coomassie | |||
| L-subunit | 74.2 ± 2.2 | 65.8 ± 9.4 | 41.2 ± 6.2 |
| H-subunit | 10 ± 3.3 | 18.8 ± 7.1 | 5.3 ± 2.9 |
| S-subunit | 15.8 ± 2.9 | 15.4 ± 3.7 | 53.5 ± 7.7 |
| Western-blot | |||
| L-subunit | 68.61 ± 0.34 | 65.60 ± 2.05 | 47.03 ± 2.05 |
| H-subunit | 10* | 18.16 ± 0.95 | 3.26 ± 1.94 |
| S-subunit | 21.39 ± 0.34 | 16.24 ± 1.44 | 49.71 ± 2.09 |
Intensity of bands was evaluated from coomassie stained gels (n = 4) using Adobe Photoshop as described in supplemental Methods.
As the H-subunit in serum is hardly visible by Coomassie and therefore quantifications are not very accurate, quantification of Western blots was done using 10% H-subunit of liver ferritin as reference point (n = 3).