The effect of pimozide on myeloid colony formation of CD34+ cells from CML patients and healthy donors
| CD34 source/treatment . | CFU-E . | BFU-E . | CFU-GM . | CFU-GEMM . |
|---|---|---|---|---|
| Healthy donors | ||||
| Vehicle | 59 ± 10 | 139 ± 60 | 36 ± 12 | 12 ± 7 |
| Pimozide | 57 ± 8 | 107 ± 16 | 21 ± 10 | 12 ± 7 |
| CML patients | ||||
| Vehicle | 60 ± 12 | 17 ± 13 | 12 ± 14 | 0 |
| Pimozide | 0 | 0 | 0 | 0 |
| CD34 source/treatment . | CFU-E . | BFU-E . | CFU-GM . | CFU-GEMM . |
|---|---|---|---|---|
| Healthy donors | ||||
| Vehicle | 59 ± 10 | 139 ± 60 | 36 ± 12 | 12 ± 7 |
| Pimozide | 57 ± 8 | 107 ± 16 | 21 ± 10 | 12 ± 7 |
| CML patients | ||||
| Vehicle | 60 ± 12 | 17 ± 13 | 12 ± 14 | 0 |
| Pimozide | 0 | 0 | 0 | 0 |
Cells were treated with vehicle or pimozide (5μM); then 10 000 cells were plated in triplicate and the number of each colony type was counted. Data are number of colonies (mean ± SD).
CFU-E indicates colony-forming unit-erythroid; BFU-E, burst-forming unit-erythroid; CFU-GM, colony-forming unit-granulocyte-macrophage; and CFU-GEMM, colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte.