Quantitative RT-PCR primers used in this study
| Target gene . | Accession no. . | Primer sequences . | Details . |
|---|---|---|---|
| Klf3 | NM_008453 | Forward: 5′-GAAATGTCACCCCCTTTAATGAAC-3′; reverse: 5′-CACGATGACGGAAGGATGGT-3′ | Ref. 19 |
| Gypa | NM_010369 | Forward: 5′-GCCGAATGACAAAGAAAAGTTCA-3′; reverse: 5′-TCAATAGAACTCAAAGGCACACTGT-3′ | ID 328119a1* |
| Gabpa | NM_008065 | Forward: 5′-AGCGCATCTCGTTGAAGAAG-3′; reverse: 5′-TCCTGCTCTTTTCTGTAGCCT-3′ | ID 4328119a1* |
| Fli-1 | NM_008026 | Forward: 5′-ATGGACGGGACTATTAAGGAGG-3′; reverse: 5′-GAAGCAGTCATATCTGCCTTGG-3′ | ID 6679807a1* |
| Ubb | NM_011664 | Forward: 5′-TGGCTATTAATTATTCGGTCTGCAT-3′; reverse: 5′-GCAAGTGGCTAGAGTGCAGAGTAA-3′ | Ref. 39 |
| 18S | NM_011664 | Forward: 5′-CACGGCCGGTACAGTGAAAC-3′; reverse: 5′-AGAGGAGCGAGCGACCAA-3′ | Ref. 19 |
| Eklf | NM_010635 | Quantitect primer assay; QIAGEN | Cat. no. QT01743777 |
| CD41 | NM_010575 | Quantitect primer assay; QIAGEN | Cat. no. QT00117992 |
| CD9 | NM_007657 | Quantitect primer assay; QIAGEN | Cat. no. QT01752513 |
| ϵy | NM_008221 | Forward: 5′-GCCCTCTCTCTAGCTGTCCA-3′; reverse: 5′-TGCTTTCAAGGAACAGTCCA-3′ | Ref. 40 |
| Target gene . | Accession no. . | Primer sequences . | Details . |
|---|---|---|---|
| Klf3 | NM_008453 | Forward: 5′-GAAATGTCACCCCCTTTAATGAAC-3′; reverse: 5′-CACGATGACGGAAGGATGGT-3′ | Ref. 19 |
| Gypa | NM_010369 | Forward: 5′-GCCGAATGACAAAGAAAAGTTCA-3′; reverse: 5′-TCAATAGAACTCAAAGGCACACTGT-3′ | ID 328119a1* |
| Gabpa | NM_008065 | Forward: 5′-AGCGCATCTCGTTGAAGAAG-3′; reverse: 5′-TCCTGCTCTTTTCTGTAGCCT-3′ | ID 4328119a1* |
| Fli-1 | NM_008026 | Forward: 5′-ATGGACGGGACTATTAAGGAGG-3′; reverse: 5′-GAAGCAGTCATATCTGCCTTGG-3′ | ID 6679807a1* |
| Ubb | NM_011664 | Forward: 5′-TGGCTATTAATTATTCGGTCTGCAT-3′; reverse: 5′-GCAAGTGGCTAGAGTGCAGAGTAA-3′ | Ref. 39 |
| 18S | NM_011664 | Forward: 5′-CACGGCCGGTACAGTGAAAC-3′; reverse: 5′-AGAGGAGCGAGCGACCAA-3′ | Ref. 19 |
| Eklf | NM_010635 | Quantitect primer assay; QIAGEN | Cat. no. QT01743777 |
| CD41 | NM_010575 | Quantitect primer assay; QIAGEN | Cat. no. QT00117992 |
| CD9 | NM_007657 | Quantitect primer assay; QIAGEN | Cat. no. QT01752513 |
| ϵy | NM_008221 | Forward: 5′-GCCCTCTCTCTAGCTGTCCA-3′; reverse: 5′-TGCTTTCAAGGAACAGTCCA-3′ | Ref. 40 |
From Primer Bank Database.41,42