Primers for genotyping and RT-qPCR
| Genotyping | ||
| Krox20 | Fwd WT | 5′ GCAGAAGGAACGGAAGAGCAG 3′ |
| Fwd Mutant | 5′ GGCCGCTTTTCTGGATTCAT 3′ | |
| Rev | 5′ ATCAAGGTCCTTTGCCCAGATC 3′ | |
| RT-qPCR | ||
| rpL10A | Fwd | 5′ CGCCGCAAGTTTCTGGAGAC 3′ |
| Rev | 5′ CTTGCCAGCCTTGTTTAGGC 3′ | |
| Krox20 | Fwd | 5′ TTGACCAGATGAACGGAGTG 3′ |
| Rev | 5′ CCAGAGAGGAGGTGGAAGTG 3′ | |
| Krox24 | Fwd | 5′ CCCTGACTATCTGTTTCC 3′ |
| Rev | 5′ TCTGCTTTCTTGTCCTTC 3′ | |
| M-CSF | Fwd | 5′ AGTCTGTCTTCCACCTGCTG 3′ |
| Rev | 5′ TTCCACCTGTCTGTCCTCAT 3′ | |
| cFms | Fwd | 5′ GGAGGTGGATTCCCTACCAT 3′ |
| Rev | 5′ GGCCCAGAACTGGTTGTAGA 3′ |
| Genotyping | ||
| Krox20 | Fwd WT | 5′ GCAGAAGGAACGGAAGAGCAG 3′ |
| Fwd Mutant | 5′ GGCCGCTTTTCTGGATTCAT 3′ | |
| Rev | 5′ ATCAAGGTCCTTTGCCCAGATC 3′ | |
| RT-qPCR | ||
| rpL10A | Fwd | 5′ CGCCGCAAGTTTCTGGAGAC 3′ |
| Rev | 5′ CTTGCCAGCCTTGTTTAGGC 3′ | |
| Krox20 | Fwd | 5′ TTGACCAGATGAACGGAGTG 3′ |
| Rev | 5′ CCAGAGAGGAGGTGGAAGTG 3′ | |
| Krox24 | Fwd | 5′ CCCTGACTATCTGTTTCC 3′ |
| Rev | 5′ TCTGCTTTCTTGTCCTTC 3′ | |
| M-CSF | Fwd | 5′ AGTCTGTCTTCCACCTGCTG 3′ |
| Rev | 5′ TTCCACCTGTCTGTCCTCAT 3′ | |
| cFms | Fwd | 5′ GGAGGTGGATTCCCTACCAT 3′ |
| Rev | 5′ GGCCCAGAACTGGTTGTAGA 3′ |