Results of NSG murine transplantations using MNCs (n = 12): high levels of human engraftment were observed with the clinically engrafting unit transplanted alone, the clinically nonengrafting unit transplanted alone, and with DCB combination
Experiment no. . | Mean human CD45+ percentage engraftment . | |||||
---|---|---|---|---|---|---|
Clinically engrafting unit alone . | Clinically nonengrafting unit alone . | DCB transplantation . | ||||
BM . | Spleen . | BM . | Spleen . | BM . | Spleen . | |
1 | 31 | 34 | 27 | 33 | 24 | 30 |
2 | 12 | 12 | 10 | 2 | 2 | 16 |
3 | 5 | 6 | 1 | 12 | 15 | 2 |
4 | 21 | 29 | 17 | 24 | 20 | 26 |
5 | 33 | 52 | 20 | 32 | 31 | 45 |
6 | 7 | 11 | 7 | 14 | 15 | 25 |
7 | 25 | 44 | 46 | 57 | 45 | 72 |
8 | 22 | 53 | 48 | 78 | 27 | 22 |
9 | 88 | 74 | 68 | 70 | 38 | 82 |
10 | 44 | 87 | 53 | 52 | 8 | 25 |
11 | Pre | Pre | 3 | 13 | 4 | 15 |
12 | 15 | 14 | 3 | 5 | 7 | 6 |
Experiment no. . | Mean human CD45+ percentage engraftment . | |||||
---|---|---|---|---|---|---|
Clinically engrafting unit alone . | Clinically nonengrafting unit alone . | DCB transplantation . | ||||
BM . | Spleen . | BM . | Spleen . | BM . | Spleen . | |
1 | 31 | 34 | 27 | 33 | 24 | 30 |
2 | 12 | 12 | 10 | 2 | 2 | 16 |
3 | 5 | 6 | 1 | 12 | 15 | 2 |
4 | 21 | 29 | 17 | 24 | 20 | 26 |
5 | 33 | 52 | 20 | 32 | 31 | 45 |
6 | 7 | 11 | 7 | 14 | 15 | 25 |
7 | 25 | 44 | 46 | 57 | 45 | 72 |
8 | 22 | 53 | 48 | 78 | 27 | 22 |
9 | 88 | 74 | 68 | 70 | 38 | 82 |
10 | 44 | 87 | 53 | 52 | 8 | 25 |
11 | Pre | Pre | 3 | 13 | 4 | 15 |
12 | 15 | 14 | 3 | 5 | 7 | 6 |
Mice were killed at a median of 6 weeks after transplantation.
Pre indicates premature mice mortality.