In vitro CFC and CAFC formation with each unit alone and in DCB combination: in vitro hematopoietic potential of CB units does not explain clinical unit dominance
Assay . | Plated cells . | N . | CFC or CAFC/1000 cells (mean ± SEM) . | Both units present in DCB cocultures (yes or no) . | |
---|---|---|---|---|---|
Clinically engrafting unit versus clinically nonengrafting unit . | DCB (P for comparisons with each unit cultured alone) . | ||||
CFC (GM, BFUe, GEMM) | |||||
Direct plating | MNCs | 5 | 6 ± 2 vs 4 ± 1 (P = .206) | 5 ± 1 (P = .479 and P = .061) | Yes (mean percentage of clinically engrafting unit only 53% ± 12%) |
CD34+ | 5 | 144 ± 28 vs 138 ± 29 (P = .885) | 165 ± 27 (P = .504 and P = .165) | ||
Overnight incubation | MNCs | 5 | 6 ± 2 vs 3 ± 1 (P = .196) | 5 ± 1 (P = .476 and P = .236) | Yes (mean percentage of clinically engrafting unit only 54% ± 10%) |
CD34+ | 5 | 135 ± 30 vs 127 ± 30 (P = .838) | 151 ± 28 (P = .377 and P = .297) | ||
CAFC | MNCs | 4 | 0.7 ± 0.5 vs 0.4 ± 0.05 (P = .479) | 0.6 ± 0.3 (P = .464 and P = .500) | See Table 2 |
CD34+ | 5 | 33 ± 11 vs 24 ± 9 (P = .266) | 23 ± 5 (P = .294 and P = .711) |
Assay . | Plated cells . | N . | CFC or CAFC/1000 cells (mean ± SEM) . | Both units present in DCB cocultures (yes or no) . | |
---|---|---|---|---|---|
Clinically engrafting unit versus clinically nonengrafting unit . | DCB (P for comparisons with each unit cultured alone) . | ||||
CFC (GM, BFUe, GEMM) | |||||
Direct plating | MNCs | 5 | 6 ± 2 vs 4 ± 1 (P = .206) | 5 ± 1 (P = .479 and P = .061) | Yes (mean percentage of clinically engrafting unit only 53% ± 12%) |
CD34+ | 5 | 144 ± 28 vs 138 ± 29 (P = .885) | 165 ± 27 (P = .504 and P = .165) | ||
Overnight incubation | MNCs | 5 | 6 ± 2 vs 3 ± 1 (P = .196) | 5 ± 1 (P = .476 and P = .236) | Yes (mean percentage of clinically engrafting unit only 54% ± 10%) |
CD34+ | 5 | 135 ± 30 vs 127 ± 30 (P = .838) | 151 ± 28 (P = .377 and P = .297) | ||
CAFC | MNCs | 4 | 0.7 ± 0.5 vs 0.4 ± 0.05 (P = .479) | 0.6 ± 0.3 (P = .464 and P = .500) | See Table 2 |
CD34+ | 5 | 33 ± 11 vs 24 ± 9 (P = .266) | 23 ± 5 (P = .294 and P = .711) |
Data are mean ± SEM of the total number of colonies (CFCs or CAFCs) per 1000 MNCs or CD34+ cells from independent experiments.
GEMM indicates granulocyte-erythrocyte-macrophage-megakaryocyte.