Table 1

In vitro CFC and CAFC formation with each unit alone and in DCB combination: in vitro hematopoietic potential of CB units does not explain clinical unit dominance

AssayPlated cellsNCFC or CAFC/1000 cells (mean ± SEM)
Both units present in DCB cocultures (yes or no)
Clinically engrafting unit versus clinically nonengrafting unitDCB (P for comparisons with each unit cultured alone)
CFC (GM, BFUe, GEMM)      
    Direct plating MNCs 6 ± 2 vs 4 ± 1 (P = .206) 5 ± 1 (P = .479 and P = .061) Yes (mean percentage of clinically engrafting unit only 53% ± 12%) 
 CD34+ 144 ± 28 vs 138 ± 29 (P = .885) 165 ± 27 (P = .504 and P = .165)  
    Overnight incubation MNCs 6 ± 2 vs 3 ± 1 (P = .196) 5 ± 1 (P = .476 and P = .236) Yes (mean percentage of clinically engrafting unit only 54% ± 10%) 
 CD34+ 135 ± 30 vs 127 ± 30 (P = .838) 151 ± 28 (P = .377 and P = .297)  
CAFC MNCs 0.7 ± 0.5 vs 0.4 ± 0.05 (P = .479) 0.6 ± 0.3 (P = .464 and P = .500) See Table 2  
 CD34+ 33 ± 11 vs 24 ± 9 (P = .266) 23 ± 5 (P = .294 and P = .711)  
AssayPlated cellsNCFC or CAFC/1000 cells (mean ± SEM)
Both units present in DCB cocultures (yes or no)
Clinically engrafting unit versus clinically nonengrafting unitDCB (P for comparisons with each unit cultured alone)
CFC (GM, BFUe, GEMM)      
    Direct plating MNCs 6 ± 2 vs 4 ± 1 (P = .206) 5 ± 1 (P = .479 and P = .061) Yes (mean percentage of clinically engrafting unit only 53% ± 12%) 
 CD34+ 144 ± 28 vs 138 ± 29 (P = .885) 165 ± 27 (P = .504 and P = .165)  
    Overnight incubation MNCs 6 ± 2 vs 3 ± 1 (P = .196) 5 ± 1 (P = .476 and P = .236) Yes (mean percentage of clinically engrafting unit only 54% ± 10%) 
 CD34+ 135 ± 30 vs 127 ± 30 (P = .838) 151 ± 28 (P = .377 and P = .297)  
CAFC MNCs 0.7 ± 0.5 vs 0.4 ± 0.05 (P = .479) 0.6 ± 0.3 (P = .464 and P = .500) See Table 2  
 CD34+ 33 ± 11 vs 24 ± 9 (P = .266) 23 ± 5 (P = .294 and P = .711)  

Data are mean ± SEM of the total number of colonies (CFCs or CAFCs) per 1000 MNCs or CD34+ cells from independent experiments.

GEMM indicates granulocyte-erythrocyte-macrophage-megakaryocyte.

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