Table 3 Summary of serial... | American Society of Hematology

Table 3

Summary of serial transplantations experiments

Patient code	Engrafted cell fraction in primary recipients*	No./mice	No. of cells/mice, ×10⁶†	No. of mice with AML/no. of mice evaluated	Time of evaluation, w	% hCD45 in mice BM‡
4	CD34⁺	4/NOD	2	4/4§	6	87/15/89/87.5
10	CD34⁺	1/NOD	0.6	1/1	18	94
11	CD34⁺	1/NOD	1.6	1/1	18	3
17‖	CD34⁺	2/NOG¶	0.125¶	1/2	25	0.12
17‖	CD34⁺	4/NOG#	0.125#	0/4	25	0
22R	CD34⁺	4/NOG	0.52	4/4	11	7.1/6/8.3/7
8R	CD34⁻ (pattern 2)	3/NOD	1	3/3	6.5	6.7/1.2/0.5
23	CD34⁻ (pattern 2)	3/NOG	4/4/2	3/3	12	60.5/79/69.5
22	CD34⁻ (pattern 3)	2/NOG	2.5	0/2	8	0
22	CD34⁻ (pattern 3)	2/NOG	1	0/2	12	0
27	CD34⁻ (pattern 3)	1/NOG	0.5	0/1	12	0

Patient code	Engrafted cell fraction in primary recipients*	No./mice	No. of cells/mice, ×10⁶†	No. of mice with AML/no. of mice evaluated	Time of evaluation, w	% hCD45 in mice BM‡
4	CD34⁺	4/NOD	2	4/4§	6	87/15/89/87.5
10	CD34⁺	1/NOD	0.6	1/1	18	94
11	CD34⁺	1/NOD	1.6	1/1	18	3
17‖	CD34⁺	2/NOG¶	0.125¶	1/2	25	0.12
17‖	CD34⁺	4/NOG#	0.125#	0/4	25	0
22R	CD34⁺	4/NOG	0.52	4/4	11	7.1/6/8.3/7
8R	CD34⁻ (pattern 2)	3/NOD	1	3/3	6.5	6.7/1.2/0.5
23	CD34⁻ (pattern 2)	3/NOG	4/4/2	3/3	12	60.5/79/69.5
22	CD34⁻ (pattern 3)	2/NOG	2.5	0/2	8	0
22	CD34⁻ (pattern 3)	2/NOG	1	0/2	12	0
27	CD34⁻ (pattern 3)	1/NOG	0.5	0/1	12	0

R indicates relapse.

See Tables 2 and 4 for details on cases used for serial transplantations.

hCD45⁺ cell equivalent.

Human cells were all hCD45⁺/CD33⁺ and expressed cytoplasmic NPM1 at immunohistochemical analysis.

From these mice, we obtained engraftment up to quaternary recipients.

Human cells recovered from engrafted mice were sorted in CD34⁺ and CD34⁻ cell fractions and inoculated separately.

CD34⁺.

CD34⁻.