Table 1 Characteristics of samples from... | American Society of Hematology

Table 1

Characteristics of samples from 21 patients with NPM1-mutated AML

Patient code	Disease status	FAB	IHC (NPM)	WB	Karyotype	FLT3 status	WBC/μL	Sample source	CD34,* % (of MNCs)	IHC (CD34), %	Post-MACS* purity (% CD34⁺)	CD34⁺/CD38⁻, % (gated on CD34⁺)	MFI* (CD34⁺/CD38⁻)
1	Diagnosis	M1	NPMc⁺	+	NA	NA	20 000	Pb	23	20-30	93	25	2.62
2	Diagnosis	M5	NPMc⁺	+	46XX; t(2; 17)(p22;q25)	FLT3-ITD	31 040	Pb	5	−	90.64	94	15
2R	Relapse	M5	NPMc⁺	+	46XX; t(2; 17)(p22;q25)	FLT3-ITD	67 080	Pb	34	−	99	98.4	18.5
4	Diagnosis	NC	NPMc⁺	+	Normal	wt	26 870	Pb	60	40-50	96.3	0.16	16
7	Relapse	M5b	NPMc⁺	NA	Normal	wt	30 170	Pb	7.4	−	94	15.6	14
8R	Relapse	M4	NPMc⁺	+	Normal	wt	127 700	Pb	1.9	−	77	33.3	1.7
10	Diagnosis	M2	NPMc⁺	+	NA	FLT3-ITD	25 000	Pb	27	Rare	98.5	70.5	3.2
11	Relapse	M2	NPMc⁺	+	Normal	wt	66 790	Pb	11.8	−	96.2	70.1	5.1
12	Diagnosis	M1	NPMc⁺	+	NA	NA	116 000	Pb	21.1	Rare	96	75.7	2.8
15	Diagnosis	M4	NPMc⁺	NA	Normal	wt	28 730	Pb	1.5	−	70	37	7.47
17	Relapse	M4	NPMc⁺	+	Normal	FLT3-ITD	35 750	Pb	27	Rare	98.3	70	5.9
18	Diagnosis	M4	NPMc⁺	+	Normal	FLT3-ITD	62 440	Pb	8.7	−	95	92.5	4.5
19	Relapse	M4	NPMc⁺	+	Normal	wt	4070	BM	1.5	−	99.7§	39	5.2
22	Diagnosis	M1	NPMc⁺	+	Normal	wt	44 000	Pb	4.3	−	86.2‖	11	2
22R	Relapse	M1	NPMc⁺	+	NA	wt	10 800	Pb	72	50-60	99.9	15	2.4
23	Relapse	M2	NPMc⁺	+	Normal	wt	19 780	Pb	1.7	Rare	18.5‖	45.5	2
25	Diagnosis	M5b	NPMc⁺†	NA	NA	FLT3-ITD	48 920	BM	2.0	NA	99¶	20	8.3
26	Diagnosis	M2	NPMc⁺†	NA	46XX; 46XX; del(3)(p21)	FLT3-ITD	2600	BM	2.6	NA	99¶	2.2	7.0
30	Diagnosis	M4	NPMc⁺	+	47XX; +8	FLT3-D385	84 770	Pb	2.93	−	79	29.4	4.8 and 11.4#
31	Diagnosis	M4	NPMc⁺	+	Normal	wt	23 000	Pb	4.76	−	92.4	45	11
32	Diagnosis	M4	NPMc⁺	+	46XX; t(2;12)	FLT3-ITD	264 000	Pb	1.3	−	81/92.3§	88	6.2 and 12.4#
34	Diagnosis	M4	NA‡	+	NA	wt	150 000	Pb	5	NA	96.5	2.48	3.8 and 13.6#
35	Diagnosis	M2	NPMc⁺	+	Normal	FLT3-ITD	7600	Pb	22	−	96.4	68	4.8

Patient code	Disease status	FAB	IHC (NPM)	WB	Karyotype	FLT3 status	WBC/μL	Sample source	CD34,* % (of MNCs)	IHC (CD34), %	Post-MACS* purity (% CD34⁺)	CD34⁺/CD38⁻, % (gated on CD34⁺)	MFI* (CD34⁺/CD38⁻)
1	Diagnosis	M1	NPMc⁺	+	NA	NA	20 000	Pb	23	20-30	93	25	2.62
2	Diagnosis	M5	NPMc⁺	+	46XX; t(2; 17)(p22;q25)	FLT3-ITD	31 040	Pb	5	−	90.64	94	15
2R	Relapse	M5	NPMc⁺	+	46XX; t(2; 17)(p22;q25)	FLT3-ITD	67 080	Pb	34	−	99	98.4	18.5
4	Diagnosis	NC	NPMc⁺	+	Normal	wt	26 870	Pb	60	40-50	96.3	0.16	16
7	Relapse	M5b	NPMc⁺	NA	Normal	wt	30 170	Pb	7.4	−	94	15.6	14
8R	Relapse	M4	NPMc⁺	+	Normal	wt	127 700	Pb	1.9	−	77	33.3	1.7
10	Diagnosis	M2	NPMc⁺	+	NA	FLT3-ITD	25 000	Pb	27	Rare	98.5	70.5	3.2
11	Relapse	M2	NPMc⁺	+	Normal	wt	66 790	Pb	11.8	−	96.2	70.1	5.1
12	Diagnosis	M1	NPMc⁺	+	NA	NA	116 000	Pb	21.1	Rare	96	75.7	2.8
15	Diagnosis	M4	NPMc⁺	NA	Normal	wt	28 730	Pb	1.5	−	70	37	7.47
17	Relapse	M4	NPMc⁺	+	Normal	FLT3-ITD	35 750	Pb	27	Rare	98.3	70	5.9
18	Diagnosis	M4	NPMc⁺	+	Normal	FLT3-ITD	62 440	Pb	8.7	−	95	92.5	4.5
19	Relapse	M4	NPMc⁺	+	Normal	wt	4070	BM	1.5	−	99.7§	39	5.2
22	Diagnosis	M1	NPMc⁺	+	Normal	wt	44 000	Pb	4.3	−	86.2‖	11	2
22R	Relapse	M1	NPMc⁺	+	NA	wt	10 800	Pb	72	50-60	99.9	15	2.4
23	Relapse	M2	NPMc⁺	+	Normal	wt	19 780	Pb	1.7	Rare	18.5‖	45.5	2
25	Diagnosis	M5b	NPMc⁺†	NA	NA	FLT3-ITD	48 920	BM	2.0	NA	99¶	20	8.3
26	Diagnosis	M2	NPMc⁺†	NA	46XX; 46XX; del(3)(p21)	FLT3-ITD	2600	BM	2.6	NA	99¶	2.2	7.0
30	Diagnosis	M4	NPMc⁺	+	47XX; +8	FLT3-D385	84 770	Pb	2.93	−	79	29.4	4.8 and 11.4#
31	Diagnosis	M4	NPMc⁺	+	Normal	wt	23 000	Pb	4.76	−	92.4	45	11
32	Diagnosis	M4	NPMc⁺	+	46XX; t(2;12)	FLT3-ITD	264 000	Pb	1.3	−	81/92.3§	88	6.2 and 12.4#
34	Diagnosis	M4	NA‡	+	NA	wt	150 000	Pb	5	NA	96.5	2.48	3.8 and 13.6#
35	Diagnosis	M2	NPMc⁺	+	Normal	FLT3-ITD	7600	Pb	22	−	96.4	68	4.8

Characteristics of other patients are shown in supplemental Table 1.

FAB indicates French-American-British classification; IHC, immunohistochemistry analysis; WB, Western blot with specific anti-NPM mutant antibodies²⁴; WBC, white blood cell; MFI, mean fluorescence intensity; NC, not classified; NA, not available; wt, wild-type; Pb, peripheral blood; R, relapse; –, CD34⁺ cells not detectable at IHC; and BM, bone marrow.

Analyzed on Cytomics FC500 cytometer equipped with the CXP analysis software (Beckman Coulter).

†

Cytoplasmic NPM was detected by flow cytometry.²³

‡

Only studied by WB.

After 2 purification steps.

‖

CD34⁺ MACS-enriched cell fraction used for FACS sorting.

CD34⁺ FACS sorted.

Two distinct cell populations (CD34^low and CD34^bright).

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