Table 1 DNA-dependent ATPase activity of... | American Society of Hematology

Table 1

DNA-dependent ATPase activity of FANCJ-WT and mutants

	K_m (mM)*	k_cat (sec⁻¹)*†	k_cat/K_m sec⁻¹ mM⁻¹	K_eff (nM) M13 ssDNA†
FANCJ-WT	0.88 ± 0.1‡	14.9 ± 1.2§	16.9	0.079 ± 0.002‡
FANCJ-A349P	0.73 ± 0.1‡	26.3 ± 2.4§	36.0	0.086 ± 0.004‡
FANCJ-K52R	ND	0.0775 ± 0.0108	ND	ND

	K_m (mM)*	k_cat (sec⁻¹)*†	k_cat/K_m sec⁻¹ mM⁻¹	K_eff (nM) M13 ssDNA†
FANCJ-WT	0.88 ± 0.1‡	14.9 ± 1.2§	16.9	0.079 ± 0.002‡
FANCJ-A349P	0.73 ± 0.1‡	26.3 ± 2.4§	36.0	0.086 ± 0.004‡
FANCJ-K52R	ND	0.0775 ± 0.0108	ND	ND

See supplemental Methods for details.

ND indicates none detectable.

M13mp18 ssDNA concentration was 2.1nM.

ATP concentration was 8.5mM.

P < .05.

P < .01.