Characterization of EA.hy926 cell and HUVEC types
| Protein . | EA.hy926 cells . | HUVECs . | |||
|---|---|---|---|---|---|
| Molecules/cell . | Molar concentration for 5 × 105 cells/mL solution, M . | Molecules/cell . | Molar concentration for 5 × 105 cells/mL solution, M . | Reference* . | |
| VWF | 2.5 × 105 | 2.0 × 10−10 | 2.6 × 105 | 2.2 × 10−10 | 58 |
| TM | 8.7 × 104 | 7.3 × 10−11 | 5 × 104, 1 × 105 | 4.2 × 10−11, 8.3 × 10−11 | 56, 85 |
| EPCR | 3.9 × 105 | 3.2 × 10−10 | 7 × 105 | 5.8 × 10−10 | 85 |
| TFPI | 2.6 × 105 | 2.2 × 10−10 | 7.8 × 104 | 6.5 × 10−11 | 57 |
| LPS-induced Tf | ND | ND | 5.0 × 103 | 4 × 10−12 | 68 |
| TNFα-induced Tf | <6.0 × 102 | <4.9 × 10−13 | 2.5 × 104 | 2.0 × 10−11 | 69 |
| Protein . | EA.hy926 cells . | HUVECs . | |||
|---|---|---|---|---|---|
| Molecules/cell . | Molar concentration for 5 × 105 cells/mL solution, M . | Molecules/cell . | Molar concentration for 5 × 105 cells/mL solution, M . | Reference* . | |
| VWF | 2.5 × 105 | 2.0 × 10−10 | 2.6 × 105 | 2.2 × 10−10 | 58 |
| TM | 8.7 × 104 | 7.3 × 10−11 | 5 × 104, 1 × 105 | 4.2 × 10−11, 8.3 × 10−11 | 56, 85 |
| EPCR | 3.9 × 105 | 3.2 × 10−10 | 7 × 105 | 5.8 × 10−10 | 85 |
| TFPI | 2.6 × 105 | 2.2 × 10−10 | 7.8 × 104 | 6.5 × 10−11 | 57 |
| LPS-induced Tf | ND | ND | 5.0 × 103 | 4 × 10−12 | 68 |
| TNFα-induced Tf | <6.0 × 102 | <4.9 × 10−13 | 2.5 × 104 | 2.0 × 10−11 | 69 |
Data are expressed in concentrations based on 5 ×105 EA.hy926 cells/mL present in the whole-blood model. EA.hy926 cells were stimulated with 250 ng/mL LPS for 4 hours or 10 ng/mL TNF-α for 4 hours in attempts to elicit expression of Tf. All other values reflect nonstimulated conditions and total expression.
Reference data for HUVECs only.
ND indicates not detected.