Summary of conditions tested during in vitro and in vivo experiments
| . | In vitro studies . | In vivo studies . |
|---|---|---|
| Cell species | (a) Human | Human |
| (b) Macaque | ||
| Conditions compared | (a) Combination of HOXB4 + DL versus HOXB4 alone and DL alone | Combination of HOXB4 + DL versus HOXB4 alone and DL alone |
| (b) Conditioned media versus coculture | ||
| DL form | (a) Conditioned media from DL-expressing OP9 cells | 2.5 μg/mL immobilized DL protein |
| (b) Coculture on feeder layer of DL-expressing OP9 cells | ||
| Percent CD34 purity after enrichment | (a) Human: average purity of 90% (n = 3) | Human: average purity of 87% (n = 5) |
| (b) Macaque: average purity of 94% (n = 3) |
| . | In vitro studies . | In vivo studies . |
|---|---|---|
| Cell species | (a) Human | Human |
| (b) Macaque | ||
| Conditions compared | (a) Combination of HOXB4 + DL versus HOXB4 alone and DL alone | Combination of HOXB4 + DL versus HOXB4 alone and DL alone |
| (b) Conditioned media versus coculture | ||
| DL form | (a) Conditioned media from DL-expressing OP9 cells | 2.5 μg/mL immobilized DL protein |
| (b) Coculture on feeder layer of DL-expressing OP9 cells | ||
| Percent CD34 purity after enrichment | (a) Human: average purity of 90% (n = 3) | Human: average purity of 87% (n = 5) |
| (b) Macaque: average purity of 94% (n = 3) |