Determination of CRU frequency and homing efficiency of B6.SJL-derived cells transplanted into B6 recipients
| . | Proportion of positive mice* . | ||
|---|---|---|---|
| 5 weeks . | 10-12 weeks . | 15-17 weeks . | |
| No. of 1° BM cells injected per mouse | |||
| 2 × 103 | 0 of 16 | 2 of 15 | 0 of 12 |
| 6 × 103 | 1 of 14 | 2 of 14 | 3 of 14 |
| 2 × 104 | 9 of 16 | 7 of 15 | 5 of 15 |
| 6 × 104 | 10 of 13 | 10 of 12 | 9 of 11 |
| 1/CRU frequency,† ± SE | 36 838 (29 084-46 660) | 31 335 (24 723-39 715) | 39 318 (30 509-50 670) |
| No. of CRUs injected,‡ ± SE | 54.3 (42.9-68.8) | 63.8 (50.4-80.9) | 50.9 (39.5-65.6) |
| Proportion of 1° BM injected per mouse | |||
| 0.005 | 0 of 15 | 3 of 11 | 0 of 8 |
| 0.014 | 1 of 14 | 2 of 12 | 0 of 12 |
| 0.042 | 0 of 12 | 2 of 11 | 1 of 11 |
| 0.125 | 2 of 13 | 4 of 12 | 1 of 9 |
| 0.16 | 4 of 13 | 5 of 12 | 3 of 12 |
| 0.2 | 3 of 11 | 3 of 9 | 5 of 9 |
| 1/CRU frequency per primary BM fraction,† ± SE | 0.59 (0.43-0.81) | 0.3 (0.2-0.3) | 0.5 (0.3-0.6) |
| CRUs recovered per primary BM,‡ ± SE | 1.7 (1.2-2.3) | 3.3 (3.3-5) | 2 (1.7-3.3) |
| Homing efficiency,§ % (CRUs recovered/CRUs injected × 100) | 3.10 | 5.20 | 3.90 |
| . | Proportion of positive mice* . | ||
|---|---|---|---|
| 5 weeks . | 10-12 weeks . | 15-17 weeks . | |
| No. of 1° BM cells injected per mouse | |||
| 2 × 103 | 0 of 16 | 2 of 15 | 0 of 12 |
| 6 × 103 | 1 of 14 | 2 of 14 | 3 of 14 |
| 2 × 104 | 9 of 16 | 7 of 15 | 5 of 15 |
| 6 × 104 | 10 of 13 | 10 of 12 | 9 of 11 |
| 1/CRU frequency,† ± SE | 36 838 (29 084-46 660) | 31 335 (24 723-39 715) | 39 318 (30 509-50 670) |
| No. of CRUs injected,‡ ± SE | 54.3 (42.9-68.8) | 63.8 (50.4-80.9) | 50.9 (39.5-65.6) |
| Proportion of 1° BM injected per mouse | |||
| 0.005 | 0 of 15 | 3 of 11 | 0 of 8 |
| 0.014 | 1 of 14 | 2 of 12 | 0 of 12 |
| 0.042 | 0 of 12 | 2 of 11 | 1 of 11 |
| 0.125 | 2 of 13 | 4 of 12 | 1 of 9 |
| 0.16 | 4 of 13 | 5 of 12 | 3 of 12 |
| 0.2 | 3 of 11 | 3 of 9 | 5 of 9 |
| 1/CRU frequency per primary BM fraction,† ± SE | 0.59 (0.43-0.81) | 0.3 (0.2-0.3) | 0.5 (0.3-0.6) |
| CRUs recovered per primary BM,‡ ± SE | 1.7 (1.2-2.3) | 3.3 (3.3-5) | 2 (1.7-3.3) |
| Homing efficiency,§ % (CRUs recovered/CRUs injected × 100) | 3.10 | 5.20 | 3.90 |
CRU indicates competitive repopulating unit; BM, bone marrow; and 1°, primary.
Recipients were considered positive if at least 5% of red cell-depleted peripheral blood cells were of donor origin (Ly-5.1).
CRU frequency is the number of BM cells containing 1 CRU.
Absolute numbers of CRUs were determined by multiplying 1 CRU frequency by the number of cells injected.
Homing efficiency is the ratio of CRUs detected in secondary recipients over the number of CRUs transplanted into the primary recipients multiplied by 100.