Eosinophil abundance relative to other leukocytes in adult zebrafish
| Organ . | n . | Leukocytes,* % (SD) . | ||
|---|---|---|---|---|
| Manual count, neutrophils (MPX+ PAS−) . | Manual count, eosinophils (MPX− PAS+) . | Flow cytometry, gata2hi cells . | ||
| Kidney | 26 | 50.2 (10.0) | 5.9 (2.2) | 4.9 (1.6) |
| IPEX | 10 | 3.4 (3.1) | 56.7 (6.2) | 51.5 (6.0) |
| Organ . | n . | Leukocytes,* % (SD) . | ||
|---|---|---|---|---|
| Manual count, neutrophils (MPX+ PAS−) . | Manual count, eosinophils (MPX− PAS+) . | Flow cytometry, gata2hi cells . | ||
| Kidney | 26 | 50.2 (10.0) | 5.9 (2.2) | 4.9 (1.6) |
| IPEX | 10 | 3.4 (3.1) | 56.7 (6.2) | 51.5 (6.0) |
Data are shown as mean ± SD. Average kidney (WKM) cell counts were 9.0 ± 1.8 × 105 (n = 5), and IPEX counts were 1.1 ± 0.3 × 105 (n = 5).
IPEX indicates intraperitoneal exudate; MPX, myeloperoxidase; and PAS, periodic acid–Schiff.
Manual count percentages were determined from single-cell suspensions of different organs stained with both MPX and PAS (500 leukocytes per cytospin); flow cytometry percentages were obtained by calculating the percentage of GFPhi cells among leukocytes.