Knockdown of galectin-1 in MSCs resulted in an altered cytokine levels in a coculture with stimulated PBMCs
| . | IFNγ . | TNFα . | IL-2 . | IL-4 . | IL-5 . | IL-10 . | IL-12 . | IL-13 . |
|---|---|---|---|---|---|---|---|---|
| IL-2/OKT3 | ||||||||
| w/o MSC | 13656 ± 1673 | 3533 ± 524 | 18.5 ± 1.8 | 684 ± 61 | 1353 ± 328 | 9.0 ± 1.0 | 368 ± 35 | |
| MSCwt | 2191 ± 1396 | 162 ± 2 | 6.2 ± 1.3 | 176 ± 29 | 651 ± 104 | 1.9 ± 1.6 | 250 ± 25 | |
| MSCcontr | 2998 ± 2010 | 182 ± 2 | 9.2 ± 1.9 | 263 ± 33 | 726 ± 64 | 2.9 ± 1.1 | 259 ± 28 | |
| MSCG1▾ | 10868* ± 2504 | 647* ± 9 | 10.8 ± 1.4 | 433 ± 117 | 1173* ± 108 | 5.7 ± 1.5 | 334 ± 54 | |
| aCD28/OKT3 | ||||||||
| w/o MSC | 8487 ± 1381 | 946 ± 228 | 42.0 ± 32.7 | 10.0 ± 1.6 | 1463 ± 176 | 235 ± 33 | 390 ± 2 | |
| MSCwt | 3305 ± 2351 | 63 ± 13 | 29.1 ± 2.5 | 14.8 ± 2.2 | 628 ± 81 | 353 ± 48 | 1469 ± 90 | |
| MSCcontr | 3655 ± 1382 | 84 ± 5 | 37.8 ± 0.6 | 13.7 ± 0.8 | 590 ± 57 | 357 ± 51 | 1051 ± 71 | |
| MSCG1▾ | 7579 ± 2770 | 180* ± 26 | 14.5* ± 2.0 | 10.7* ± 1.0 | 1300* ± 248 | 495* ± 64 | 1050 ± 124 |
| . | IFNγ . | TNFα . | IL-2 . | IL-4 . | IL-5 . | IL-10 . | IL-12 . | IL-13 . |
|---|---|---|---|---|---|---|---|---|
| IL-2/OKT3 | ||||||||
| w/o MSC | 13656 ± 1673 | 3533 ± 524 | 18.5 ± 1.8 | 684 ± 61 | 1353 ± 328 | 9.0 ± 1.0 | 368 ± 35 | |
| MSCwt | 2191 ± 1396 | 162 ± 2 | 6.2 ± 1.3 | 176 ± 29 | 651 ± 104 | 1.9 ± 1.6 | 250 ± 25 | |
| MSCcontr | 2998 ± 2010 | 182 ± 2 | 9.2 ± 1.9 | 263 ± 33 | 726 ± 64 | 2.9 ± 1.1 | 259 ± 28 | |
| MSCG1▾ | 10868* ± 2504 | 647* ± 9 | 10.8 ± 1.4 | 433 ± 117 | 1173* ± 108 | 5.7 ± 1.5 | 334 ± 54 | |
| aCD28/OKT3 | ||||||||
| w/o MSC | 8487 ± 1381 | 946 ± 228 | 42.0 ± 32.7 | 10.0 ± 1.6 | 1463 ± 176 | 235 ± 33 | 390 ± 2 | |
| MSCwt | 3305 ± 2351 | 63 ± 13 | 29.1 ± 2.5 | 14.8 ± 2.2 | 628 ± 81 | 353 ± 48 | 1469 ± 90 | |
| MSCcontr | 3655 ± 1382 | 84 ± 5 | 37.8 ± 0.6 | 13.7 ± 0.8 | 590 ± 57 | 357 ± 51 | 1051 ± 71 | |
| MSCG1▾ | 7579 ± 2770 | 180* ± 26 | 14.5* ± 2.0 | 10.7* ± 1.0 | 1300* ± 248 | 495* ± 64 | 1050 ± 124 |
MSCwt, MSCcontr, and MSCG1▾ were cocultured with PBMC either stimulated with IL-2/OKT3 or αCD28/OKT3. The cytokine levels were determined by Bio-Plex Pro Assays and are shown in picograms per milliliter. Knockdown of galectin-1 resulted in an increase of the proinflammatory cytokines IFNγ and TNFα. Data are shown as means of triplicates (± SD).
MSC indicates mesenchymal stromal cell; and PBMC, peripheral blood mononuclear cell.
Significant difference between MSCcontr and MSCG1▾ (P < .05).