Oligonucleotides used to generate retroviral plasmids for RNA silencing
| . | Oligonucleotide . |
|---|---|
| Galectin-1 | |
| Forward | 5′-gatccccGCTGCCAGATGGATACGAAttcaagagaTTCGTATCCATCTGGCAGCtttttggaaa-3′ |
| Reverse | 5′-cgatttccaaaaaGCTGCCAGATGGATACGAAtctcttgaaTTCGTATCCATCTGGCAGCggg-3′ |
| Luciferase | |
| Forward | 5′-gatccccCGTACGCGGAATACTTCGAttcaagagaTCGAAGTATTCCGCGTACGtttttggaaa-3′ |
| Reverse | 5′-cgatttccaaaaaCGTACGCGGAATACTTCGAtctcttgaaTCGAAGTATTCCGCGTACGggg-3′ |
| AML/MTG8 fusion protein | |
| Forward | 5′-gatccccCCTCGAAATCGTACTGAGAttcaagagaTCTCAGTACGATTTCGAGGtttttggaaa-3′ |
| Reverse | 5′-cgatttccaaaaaCCTCGAAATCGTACTGAGAtctcttgaaTCTCAGTACGATTTCGAGGggg-3′ |
| . | Oligonucleotide . |
|---|---|
| Galectin-1 | |
| Forward | 5′-gatccccGCTGCCAGATGGATACGAAttcaagagaTTCGTATCCATCTGGCAGCtttttggaaa-3′ |
| Reverse | 5′-cgatttccaaaaaGCTGCCAGATGGATACGAAtctcttgaaTTCGTATCCATCTGGCAGCggg-3′ |
| Luciferase | |
| Forward | 5′-gatccccCGTACGCGGAATACTTCGAttcaagagaTCGAAGTATTCCGCGTACGtttttggaaa-3′ |
| Reverse | 5′-cgatttccaaaaaCGTACGCGGAATACTTCGAtctcttgaaTCGAAGTATTCCGCGTACGggg-3′ |
| AML/MTG8 fusion protein | |
| Forward | 5′-gatccccCCTCGAAATCGTACTGAGAttcaagagaTCTCAGTACGATTTCGAGGtttttggaaa-3′ |
| Reverse | 5′-cgatttccaaaaaCCTCGAAATCGTACTGAGAtctcttgaaTCTCAGTACGATTTCGAGGggg-3′ |
AML/MTG8 indicates fusion protein for acute myeloid leukemia.