Summary of T-ALL engraftment before and after coculture with MS5 cells
| . | NOD-Scid recipients (positive/total) . | ||
|---|---|---|---|
| I mice . | II mice . | III mice . | |
| Patients | 15/15 | 10/10 | ND |
| MS5-DL1/−GSI | 30/30 | 21/21 | 9/9 |
| MS5-DL1/+GSI | 2/16 | 0/2 | — |
| MS5-GFP/−GSI | 1/12* | 0/1* | — |
| 11/13† | 5/8† | ND | |
| . | NOD-Scid recipients (positive/total) . | ||
|---|---|---|---|
| I mice . | II mice . | III mice . | |
| Patients | 15/15 | 10/10 | ND |
| MS5-DL1/−GSI | 30/30 | 21/21 | 9/9 |
| MS5-DL1/+GSI | 2/16 | 0/2 | — |
| MS5-GFP/−GSI | 1/12* | 0/1* | — |
| 11/13† | 5/8† | ND | |
A total of 2.5 × 105 T-ALL blasts were either injected (Patients) using IBI or cultured with MS5-GFP and MS5-DL1 (± GSI) during 30 days. Wells were then harvested and cells recovered from individual wells were transplanted using IBI into a single mouse.
— indicates not applicable; and ND, not done.
Results from M18 and M22 T-ALL.
Results from M30 T-ALL. Results from primary/I mice are shown also in Figure 5B,C. II mice and III mice are secondary and tertiary transplanted mice, respectively.