Table 1 Effects of short-term in vitro... | American Society of Hematology

Table 1

Effects of short-term in vitro exposure of SLAM SKL cells to dmPGE₂ on cell cycle

In vitro treatment	SLAM SKL cells*
In vitro treatment	G₀	G₁	S+G_2M	% cells in cycle†
Vehicle	63.4 ± 2.5	2.6 ± 0.7	33.8 ± 2.1	36.4 ± 2.4
1 μM dmPGE₂	54.8 ± 2.2‡	6.8 ± 1.9‡	38.4 ± 1.6‡	45.2 ± 2.2‡

In vitro treatment	SLAM SKL cells*
In vitro treatment	G₀	G₁	S+G_2M	% cells in cycle†
Vehicle	63.4 ± 2.5	2.6 ± 0.7	33.8 ± 2.1	36.4 ± 2.4
1 μM dmPGE₂	54.8 ± 2.2‡	6.8 ± 1.9‡	38.4 ± 1.6‡	45.2 ± 2.2‡

Lin^neg cells, treated with either 1 μM dmPGE₂ or vehicle for 2 hours and cultured in the presence of growth factors (50 ng/mL rmSCF, 100 ng/mL each of rhFlt-3 and rhTPO) for 20 hours, were stained with SLAM SKL, Hoechst-33342, and Pyronin-Y and the proportion of SLAM SKL cells in G₀, G₁, S, and G₂/M phase of the cell cycle was determined by quantitation of DNA and RNA content by FACS. Data are mean plus or minus SEM for n = 9 mice, each assayed individually.

†

Percentage of cells in G₁+S+G₂M; combined data for n = 9 mice.

‡

P < .05 compared with vehicle control.

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