The primer sequences used for RT-PCR
| Gene . | Sequence . |
|---|---|
| HAMP (forward) | 5′AGAGCTGCAGCCTTTGCAC-3′ |
| HAMP (reverse) | 5′GAAGATGCAGATGGGGAAGT-3′ |
| IL-6 (forward) | 5′-AGTTGCCTTCTTGGGACTGA-3′ |
| IL-6 (reverse) | 5′-TCCACGATTTCCCAGAGAAC-3′ |
| Actin (forward) | 5′-GACGGCCAAGTCATCACTATTG-3′ |
| Actin (reverse) | 5′-CCACAGGATTCCATACCCAAGA-3′ |
| Gene . | Sequence . |
|---|---|
| HAMP (forward) | 5′AGAGCTGCAGCCTTTGCAC-3′ |
| HAMP (reverse) | 5′GAAGATGCAGATGGGGAAGT-3′ |
| IL-6 (forward) | 5′-AGTTGCCTTCTTGGGACTGA-3′ |
| IL-6 (reverse) | 5′-TCCACGATTTCCCAGAGAAC-3′ |
| Actin (forward) | 5′-GACGGCCAAGTCATCACTATTG-3′ |
| Actin (reverse) | 5′-CCACAGGATTCCATACCCAAGA-3′ |