Differences in phenotypic marker expression by neonatal and adult RTE
| . | CD24 . | Qa2 . | αβTCR . | CD3 . | CD28 . | IL-7Rα . |
|---|---|---|---|---|---|---|
| Neonatal CD4+ | ||||||
| GFPhi RTE | 148 ± 58* | 49 ± 8* | 1158 ± 99* | 478 ± 24* | 355 ± 35* | 114 ± 11 |
| GFPlo intermediate | 89 ± 40* | 29 ± 5* | 893 ± 71* | 286 ± 17 | 208 ± 20* | 113 ± 6 |
| GFP− resident | 62 ± 34* | 25 ± 3* | 717 ± 61* | 114 ± 10* | 53 ± 6 | 103 ± 5 |
| Adult CD4+ | ||||||
| GFPhi RTE | 74 ± 5 | 76 ± 2 | 1379 ± 79 | 316 ± 91 | 133 ± 76 | 301 ± 7 |
| GFPlo intermediate | 30 ± 1 | 86 ± 2 | 1175 ± 43 | 226 ± 58 | 76 ± 53 | 262 ± 14 |
| GFP− resident | 13 ± 1 | 87 ± 4 | 1047 ± 45 | 168 ± 56 | 44 ± 26 | 197 ± 8 |
| . | CD24 . | Qa2 . | αβTCR . | CD3 . | CD28 . | IL-7Rα . |
|---|---|---|---|---|---|---|
| Neonatal CD4+ | ||||||
| GFPhi RTE | 148 ± 58* | 49 ± 8* | 1158 ± 99* | 478 ± 24* | 355 ± 35* | 114 ± 11 |
| GFPlo intermediate | 89 ± 40* | 29 ± 5* | 893 ± 71* | 286 ± 17 | 208 ± 20* | 113 ± 6 |
| GFP− resident | 62 ± 34* | 25 ± 3* | 717 ± 61* | 114 ± 10* | 53 ± 6 | 103 ± 5 |
| Adult CD4+ | ||||||
| GFPhi RTE | 74 ± 5 | 76 ± 2 | 1379 ± 79 | 316 ± 91 | 133 ± 76 | 301 ± 7 |
| GFPlo intermediate | 30 ± 1 | 86 ± 2 | 1175 ± 43 | 226 ± 58 | 76 ± 53 | 262 ± 14 |
| GFP− resident | 13 ± 1 | 87 ± 4 | 1047 ± 45 | 168 ± 56 | 44 ± 26 | 197 ± 8 |
The average mean fluorescence intensity (MFI) of the cell-surface markers shown in Figure 1E was calculated for neonatal and adult LN cells, and is shown as the mean plus or minus SD. Where 2 peaks were evident (CD24 and Qa2), the MFI was determined using the larger (main) peak. For all markers except IL-7Rα, data represent mean plus or minus SD of MFI from individual animals. CD24: n = 11 neonates and 9 adults. Qa2: n = 14 neonates and 6 adults. αβTCR, CD3, and CD28: n = 10 neonates and 8 adults. For IL-7Rα, data represent 2 independent experiments using a pool of LN cells from 14 or 15 neonates and 2 adults per experiment.
P < .005, neonatal cell subset versus respective adult cell subset.