Fetal hemoglobin induction in K562 cells by drug inducers
| Compound . | Drugs . | HbF/TH/TP* . | P . | Fold increase . |
|---|---|---|---|---|
| Untreated cells | None | 0.29 ± 0.04 | – | 1.00 |
| Hemin | 50μM | 0.92 ± 0.09 | < .001 | 3.17 |
| Butyrate | 2mM | 0.58 ± 0.05 | .002 | 2.52 |
| Trichostatin A | 0.5μM | 1.02 ± 0.16 | .002 | 3.76 |
| SAHA | 5μM | 0.62 ± 0.06 | .002 | 2.13 |
| Compound . | Drugs . | HbF/TH/TP* . | P . | Fold increase . |
|---|---|---|---|---|
| Untreated cells | None | 0.29 ± 0.04 | – | 1.00 |
| Hemin | 50μM | 0.92 ± 0.09 | < .001 | 3.17 |
| Butyrate | 2mM | 0.58 ± 0.05 | .002 | 2.52 |
| Trichostatin A | 0.5μM | 1.02 ± 0.16 | .002 | 3.76 |
| SAHA | 5μM | 0.62 ± 0.06 | .002 | 2.13 |
Fetal hemoglobin (HbF) levels were quantified by ELISA after 48 hours of drug treatment. Readings were normalized by total hemoglobin (TH) and total protein (TP) levels measured by spectrophotometry and expressed as mean ± SD.