RT-PCR primer sequences and conditions
| PCR product . | Primer sequence (5′-3′) . | Tann . | No. of cycles . | MgCl2 concentration . |
|---|---|---|---|---|
| CCR5 | F-CTGAGACATCCGTTCCCCTA | 60°C | 30 | 3 mM |
| R-GCTCTTCAGCCTTTTGCAGT | ||||
| NF-κB p50 | F-GAACTCCTCCATTGTGGAACC | 62°C | 32 | 1.5 mM |
| R-CCCGGAGATTTGCTGTCATG | ||||
| HLA-DRA | F-GGCCATAAGTGGAGTCCC | 55°C | 30 | 3 mM |
| R-CTATACTCCGATCACCAA | ||||
| CIITA-PIV | F-AGCTGGCGGGAGGGAGAGGCCACC | 60°C | 35 | 1.75 mM |
| R-CATACTGGTCCAGTTCCGCGATATTGG | ||||
| ICER | F-CAGATCCGAGCTCCTACTGC | 60°C | 27 | 3 mM |
| R-CAACTCGGCTCTCCAGACAT | ||||
| CREB-1 | F-AACCAGCAGAGTGGAGATGCAGCT | 60°C | 30 | 4 mM |
| R-CTGTAGGAAGGCCTCCTTGAAAGA | ||||
| GAPDH | F-GGTCGGAGTCAACGGATTTG | 60°C | 22 | 1.5 mM |
| R-ATGAGCCCCAGCCTTCTCCAT |
| PCR product . | Primer sequence (5′-3′) . | Tann . | No. of cycles . | MgCl2 concentration . |
|---|---|---|---|---|
| CCR5 | F-CTGAGACATCCGTTCCCCTA | 60°C | 30 | 3 mM |
| R-GCTCTTCAGCCTTTTGCAGT | ||||
| NF-κB p50 | F-GAACTCCTCCATTGTGGAACC | 62°C | 32 | 1.5 mM |
| R-CCCGGAGATTTGCTGTCATG | ||||
| HLA-DRA | F-GGCCATAAGTGGAGTCCC | 55°C | 30 | 3 mM |
| R-CTATACTCCGATCACCAA | ||||
| CIITA-PIV | F-AGCTGGCGGGAGGGAGAGGCCACC | 60°C | 35 | 1.75 mM |
| R-CATACTGGTCCAGTTCCGCGATATTGG | ||||
| ICER | F-CAGATCCGAGCTCCTACTGC | 60°C | 27 | 3 mM |
| R-CAACTCGGCTCTCCAGACAT | ||||
| CREB-1 | F-AACCAGCAGAGTGGAGATGCAGCT | 60°C | 30 | 4 mM |
| R-CTGTAGGAAGGCCTCCTTGAAAGA | ||||
| GAPDH | F-GGTCGGAGTCAACGGATTTG | 60°C | 22 | 1.5 mM |
| R-ATGAGCCCCAGCCTTCTCCAT |
Tann indicates annealing temperature.