Table 1

ds-oligonucleotide sequences used for EMSA

EMSA probeOligonucleotide sequence*Location
ISRE β2m21 5′-TAAGAAAAGGAAACTGAAAACG-3′  
CCR5-ISRE-1 5′-CTCCGCATGGTGAAAGTAAGAACC-3′ −4026 
CCR5-ISRE-2 5′-GCAATTAGCTTTACCTTTTCAGCTTCT-3′ −3367 
CCR5-ISRE-3 5′-GGACTGCTGAAAGAGTAACTAAGAGTT-3′ −3255 
κB β2m21 5′-ACGGGAAAGTCCCTC-3′  
CCR5-κB-1 5′-GAACAGAGTGAAAATCCCCACTAAGA-3′ −2650 
CCR5-κB-2 5′-CTTACTGTTGAAAAGCCCTGTGATCT-3′ −2340 
CCR5-κB-3 5′-ATCCAGTGAGAAAAGCCCGTAAATAA-3′ −2155 
CRE consensus27 5′-AGAGATTGCCTGACGTCAGAGAGCTAG-3′  
CCR5-CRE-1 5′-AACACAAAAGTGGAGTAACGCACA-3′ −4369 
CCR5-CRE-2 5′-CAGGTCTAGCACGTCATTTAACAG-3′ −4226 
CCR5-CRE-3 5′-TATCTTGCCGAGGTCACAAAGCAA-3′ −4172 
CCR5-CRE-B1 5′-GATTGGGGGCACGTAATTTTGCTG-3′ −3028 
CCR5-CRE-B2 5′-AGCCAAGGTCACGGAAGCCCAGAG-3′ −2950 
CCR5-CRE-B3 5′-AGATTTTCAGATGTCACCAACCGC-3′ −2866 
CCR5-CRE-B4 5′-CCATATACTTATGTCATGTGGAAA-3′ −2788 
CCR5-CRE-B5 5′-GGTTAATGTGAAGTCCAGGATCCC-3′ −2445 
CCR5-CRE-B6 5′-TGGGCTTTTGACTAGATGAATGTA-3′ −2267 
CCR5-CRE-B7 5′-TAGTGGGATGAGCAGAGAACAAAA-3′ −2187 
CCR5-CRE-B8 5′-GCTTATTTTAAGCTCAACTTAAAA-3′ −2102 
CCR5-CRE-B9 5′-TCTAGCTCTGATATCCTTTATTCT-3′ −2235 
CCR5-CRE-B10 5′-CGTAAATAAACCTTCAGACCAGAG-3′ −2138 
CCR5-CRE-B11 5′-ATTCTTTTCGCCTTCAATACACTT-3′ −2061 
CCR5-CRE-B12 5′-ACTCCACCCTCCTTCAAAAGAAAC-3′ −2028 
CCR5-CRE-B13 5′-TGATTTGCACAGCTCATCTGGCCA-3′ −1972 
EMSA probeOligonucleotide sequence*Location
ISRE β2m21 5′-TAAGAAAAGGAAACTGAAAACG-3′  
CCR5-ISRE-1 5′-CTCCGCATGGTGAAAGTAAGAACC-3′ −4026 
CCR5-ISRE-2 5′-GCAATTAGCTTTACCTTTTCAGCTTCT-3′ −3367 
CCR5-ISRE-3 5′-GGACTGCTGAAAGAGTAACTAAGAGTT-3′ −3255 
κB β2m21 5′-ACGGGAAAGTCCCTC-3′  
CCR5-κB-1 5′-GAACAGAGTGAAAATCCCCACTAAGA-3′ −2650 
CCR5-κB-2 5′-CTTACTGTTGAAAAGCCCTGTGATCT-3′ −2340 
CCR5-κB-3 5′-ATCCAGTGAGAAAAGCCCGTAAATAA-3′ −2155 
CRE consensus27 5′-AGAGATTGCCTGACGTCAGAGAGCTAG-3′  
CCR5-CRE-1 5′-AACACAAAAGTGGAGTAACGCACA-3′ −4369 
CCR5-CRE-2 5′-CAGGTCTAGCACGTCATTTAACAG-3′ −4226 
CCR5-CRE-3 5′-TATCTTGCCGAGGTCACAAAGCAA-3′ −4172 
CCR5-CRE-B1 5′-GATTGGGGGCACGTAATTTTGCTG-3′ −3028 
CCR5-CRE-B2 5′-AGCCAAGGTCACGGAAGCCCAGAG-3′ −2950 
CCR5-CRE-B3 5′-AGATTTTCAGATGTCACCAACCGC-3′ −2866 
CCR5-CRE-B4 5′-CCATATACTTATGTCATGTGGAAA-3′ −2788 
CCR5-CRE-B5 5′-GGTTAATGTGAAGTCCAGGATCCC-3′ −2445 
CCR5-CRE-B6 5′-TGGGCTTTTGACTAGATGAATGTA-3′ −2267 
CCR5-CRE-B7 5′-TAGTGGGATGAGCAGAGAACAAAA-3′ −2187 
CCR5-CRE-B8 5′-GCTTATTTTAAGCTCAACTTAAAA-3′ −2102 
CCR5-CRE-B9 5′-TCTAGCTCTGATATCCTTTATTCT-3′ −2235 
CCR5-CRE-B10 5′-CGTAAATAAACCTTCAGACCAGAG-3′ −2138 
CCR5-CRE-B11 5′-ATTCTTTTCGCCTTCAATACACTT-3′ −2061 
CCR5-CRE-B12 5′-ACTCCACCCTCCTTCAAAAGAAAC-3′ −2028 
CCR5-CRE-B13 5′-TGATTTGCACAGCTCATCTGGCCA-3′ −1972 
*

Potential protein binding sites are underlined.

Location relative to start ORF (+1) according to numbering proposed at the 1999 CCR5-AIDS symposium (based before on accession nos. AF031236 and AF031237).26,34 

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