The EC50 (± SEM) for DHG inhibition of the mean velocity index for thrombin generation (μM) in immunodepleted plasma
| TF, pM . | Mock-depleted plasma . | HCII-depleted plasma . |
|---|---|---|
| 0.2 | 0.16 ± 0.01 | 0.16 ± 0.01 |
| 4 | 2.02 ± 0.09 | 4.31 ± 0.23 |
| TF, pM . | Mock-depleted plasma . | HCII-depleted plasma . |
|---|---|---|
| 0.2 | 0.16 ± 0.01 | 0.16 ± 0.01 |
| 4 | 2.02 ± 0.09 | 4.31 ± 0.23 |
Thrombin generation was initiated with 0.2 or 4 pM human TF, 8.3 μM PC:PS vesicles, and 40 μg/mL CTI (plasma concentrations) in mock- or HCII-depleted plasma in the presence of increasing DHG (0-10 μM). Duplicate wells were averaged to determine individual thrombin generation curves. The relative velocity index was plotted versus DHG concentration, and EC50 values for each replicate (n = 3) were determined as described in “Fluorogenic assay for detection of plasma thrombin generation.” The mean and standard error of the EC50 values were determined from the 3 independent replicates.
Statistical significance analysis: EC50 values for DHG inhibition at 4 pM TF were significantly different from 0.2 pM in both plasma. At 4 pM TF, EC50 value in HCII-depleted plasma was significantly different from mock-depleted plasma (Student t test, P < .002).