List of differentially abundant protein spots with corresponding average ratio and DeCyder P values
| Spot no. . | Protein . | Average ratio . | DeCyder P . | SC, % . | IS . | PS . | Accession no. . | No. of identified peptides . | Mass database, Da . | Mass gel, Da . | pI database . | pI gel . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Ig gamma-1 chain C region (rat) | 5.3 | 1.3e−05 | 37 | 728 | 23 | 36 493 | 55 000 | 6.43 | 5.6 | ||
| 2 | Ig gamma-1 chain C region (rat) | 4.8 | 4.2e−11 | 26 | 380 | 10 | 35 923 | 55 000 | 6.43 | 5.9 | ||
| 3 | Ig gamma-1 chain C region (rat) | 3.0 | 3.8e−7 | 26 | 626 | P30101 | 22 | 36 493 | 55 000 | 6.43 | 5.9 | |
| ERp57 | 10 | 110 | P30101 | 3 | 57 146 | 5.98 | ||||||
| 4 | Src substrate cortactin | 2.4 | 1.2e−08 | 37 | 97 | 209 | Q14247 | 1 | 61 770 | 50 000 | 5.24 | 6.1 |
| 5 | Pleckstrin | 6.6 | 7.4e−03 | 24 | 189 | 223 | P08567 | 3 | 40 456 | 45 000 | 8.32 | 6.0 |
| 6 | Pleckstrin | 5.4 | 9.8e−03 | 43 | 371 | 459 | P08567 | 4 | 40 456 | 45 000 | 8.50 | 6.1 |
| 7 | Pleckstrin | 5.0 | 1.2e−02 | 22 | 302 | 328 | P08567 | 4 | 40 456 | 45 000 | 8.50 | 6.2 |
| beta-centractin | 35 | 64 | 143 | P42025 | 1 | 42 381 | 5.98 | |||||
| 8 | Charged multivesicular body protein 3 | 10.6 | 8.5e−12 | 16 | 122 | Q9CQ10 | 3 | 24 983 | 29 000 | 5.10 | 5.3 | |
| 9 | Pyruvate kinase isozymes M1/M2 | −1.6 | 3.2e−04 | 50 | 355 | 559 | P14618 | 7 | 58 339 | 55 000 | 7.95 | 7.5 |
| N(2) N(2)-dimethylguanosine tRNA methyltransferase | 16 | 11 | 59 | Q9NXH9 | 0 | 73 386 | 7.77 | |||||
| 10 | Pleckstrin | −1.5 | 2.7e−02 | 37 | 426 | 499 | P08567 | 6 | 40 471 | 45 000 | 8.50 | 6.6 |
| 11 | Pleckstrin | −1.6 | 3.8e−02 | 30 | 231 | 275 | P08567 | 4 | 40 471 | 45 000 | 8.50 | 6.9 |
| 12 | Pleckstrin | −1.5 | 1.1e−02 | 30 | 177 | 226 | P08567 | 3 | 40 471 | 45 000 | 8.50 | 7.5 |
| 13 | Aldose reductase | 1.5 | 4.6e−02 | 14 | 448 | P45376 | 32 | 36 099 | 36 000 | 6.56 | 6.4 |
| Spot no. . | Protein . | Average ratio . | DeCyder P . | SC, % . | IS . | PS . | Accession no. . | No. of identified peptides . | Mass database, Da . | Mass gel, Da . | pI database . | pI gel . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Ig gamma-1 chain C region (rat) | 5.3 | 1.3e−05 | 37 | 728 | 23 | 36 493 | 55 000 | 6.43 | 5.6 | ||
| 2 | Ig gamma-1 chain C region (rat) | 4.8 | 4.2e−11 | 26 | 380 | 10 | 35 923 | 55 000 | 6.43 | 5.9 | ||
| 3 | Ig gamma-1 chain C region (rat) | 3.0 | 3.8e−7 | 26 | 626 | P30101 | 22 | 36 493 | 55 000 | 6.43 | 5.9 | |
| ERp57 | 10 | 110 | P30101 | 3 | 57 146 | 5.98 | ||||||
| 4 | Src substrate cortactin | 2.4 | 1.2e−08 | 37 | 97 | 209 | Q14247 | 1 | 61 770 | 50 000 | 5.24 | 6.1 |
| 5 | Pleckstrin | 6.6 | 7.4e−03 | 24 | 189 | 223 | P08567 | 3 | 40 456 | 45 000 | 8.32 | 6.0 |
| 6 | Pleckstrin | 5.4 | 9.8e−03 | 43 | 371 | 459 | P08567 | 4 | 40 456 | 45 000 | 8.50 | 6.1 |
| 7 | Pleckstrin | 5.0 | 1.2e−02 | 22 | 302 | 328 | P08567 | 4 | 40 456 | 45 000 | 8.50 | 6.2 |
| beta-centractin | 35 | 64 | 143 | P42025 | 1 | 42 381 | 5.98 | |||||
| 8 | Charged multivesicular body protein 3 | 10.6 | 8.5e−12 | 16 | 122 | Q9CQ10 | 3 | 24 983 | 29 000 | 5.10 | 5.3 | |
| 9 | Pyruvate kinase isozymes M1/M2 | −1.6 | 3.2e−04 | 50 | 355 | 559 | P14618 | 7 | 58 339 | 55 000 | 7.95 | 7.5 |
| N(2) N(2)-dimethylguanosine tRNA methyltransferase | 16 | 11 | 59 | Q9NXH9 | 0 | 73 386 | 7.77 | |||||
| 10 | Pleckstrin | −1.5 | 2.7e−02 | 37 | 426 | 499 | P08567 | 6 | 40 471 | 45 000 | 8.50 | 6.6 |
| 11 | Pleckstrin | −1.6 | 3.8e−02 | 30 | 231 | 275 | P08567 | 4 | 40 471 | 45 000 | 8.50 | 6.9 |
| 12 | Pleckstrin | −1.5 | 1.1e−02 | 30 | 177 | 226 | P08567 | 3 | 40 471 | 45 000 | 8.50 | 7.5 |
| 13 | Aldose reductase | 1.5 | 4.6e−02 | 14 | 448 | P45376 | 32 | 36 099 | 36 000 | 6.56 | 6.4 |
A positive algebraic sign indicates an increase of intensity in the GPVI-activated samples; a negative algebraic sign indicates a decrease. Data were generated using DeCyder software.
SC indicates sequence coverage; IS, Mascot25 ions score; and PS, Mascot25 protein score.